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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov., isolated from hypersaline Lake Pengyanco on the Tibetan Plateau Free

Abstract

A Gram-stain-negative, catalase- and oxidase-positive, aerobic, rod-shaped, motile strain (PYC7W) was isolated from Lake Pengyanco on the Tibetan Plateau. Comparisons based on 16S rRNA gene sequences showed that strain PYC7W belongs to the genus , with YU-PRIM-29 and T68687 as its closest neighbours (96.8 and 96.6 % 16S rRNA gene sequence similarity, respectively), and only 93.1 % 16S rRNA gene sequence similarity to ATCC 33173. The predominant respiratory quinone of strain PYC7W is Q-9, with Q-8 as a minor component. The major fatty acids are C 6 and / or C ω7, C, C 6 and/or C ω7, and C 3OH. The polar lipids of strain PYC7W include phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. Genome sequencing revealed a genome size of 4.79 Mbp and a G+C content of 62.9 mol%. DNA–DNA hybridization values of strain PYC7W showed 45, 30 and 38 % relatedness with DSM 21197, DSM 21196 and DSM 21198, respectively. Combining phenotypic, biochemical, genotypic and DNA–DNA hybridization data, we propose that strain PYC7W represents a novel species within the genus and to have the name sp. nov.; PYC7W (=CICC 24506= KCTC 62529) is the type strain.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Halomonas , hypersaline lake , polyphasic taxonomy and Tibetan Plateau
Funding
This study was supported by the:
  • Chinese Academy of Sciences (Award QYZDJ-SSW-DQC030)
    • Principle Award Recipient: Qinglong Wu
  • National Natural Science Foundation of China (Award 31730013)
    • Principle Award Recipient: Qinglong Wu
  • the Youth Innovation Promotion Association of CAS (Award 2014273)
    • Principle Award Recipient: Peng Xing
  • National Natural Science Foundation of China (Award 31722008)
    • Principle Award Recipient: Peng Xing
  • Science & Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Peng Xing
© 2020 Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences
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Halomonas montanilacus sp. nov., isolated from hypersaline Lake Pengyanco on the Tibetan Plateau
Int J Syst Evol Microbiol 70, 2859 (2020); https://doi.org/10.1099/ijsem.0.004109
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