1887

Abstract

A novel actinobacterium, designated TRM68348, was isolated from the silt collected from the Tailan River in Xinjiang Province, north-west China. The strain was aerobic and Gram-stain-positive. The aerial mycelium was densely straight or tortuous, with a few branches of hyphae and no spores. The whole-cell sugar pattern of strain TRM68348 consisted of ribose and glucose. The diagnostic diamino was -diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannose and an unidentified phospholipid. The predominant menaquinones were MK-9 (H), MK-9 (H) and MK-9 (H). The major fatty acids (>5 %) were iso-C, iso-C, anteiso-C, iso-C, C and summed feature 6. The G+C content of the genomic DNA was 69.93 mol%. Phylogenetic analysis showed that strain TRM68348 shared 16S rRNA gene sequence similarity of 98.14 % to the closest described species 1H-SSA4. Strain TRM68348 had a relatively low DNA–DNA relatedness value with 1H-SSA4 as determined by calculating the average nucleotide identity value (92.78 %). Strain TRM68348 could also be differentiated from 1H-SSA4 based on morphological and physiological characteristics. On the basis of the evidence from this polyphasic study, the strain is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM68348 (=CCTCC AA 2018086=KCTC 49274).

Funding
This study was supported by the:
  • Bei-Bei Sun , Tarim University graduate research and innovation projects , (Award XJ2019G270)
  • Li-Li Zhang , National Natural Science Foundation of China , (Award Xinjiang Joint Fund Key Project (U1703236))
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2020-03-20
2020-06-02
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