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Volume 70, Issue 4

sp. nov., isolated from the profound head ulcers of farmed Murray cod () Free

Abstract

A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium with monopolar flagella, designated as MC042, was isolated from the profound head ulcers of farmed Murray cod sampled from Zhejiang Province, China. Analysis of its 16S rRNA gene sequence and multilocus sequence analysis phylogeny showed that strain MC042 belonged to the genus , showing the highest 16S rRNA gene sequence similarity to BML3 (98.9 %), and less than 98.8 % similarity to other species with validly published names. Whole-genome sequencing and phylogenetic reconstruction based on a core set of 1563 genes further indicated that strain MC042 was most closely related to the clade formed by CHA0 and DSM 975 and distantly related to any of the validly published species of the genus . Furthermore, strain MC042 could be distinguished from its closely related species of the genus by its ability to assimilate maltose, -xylose and melibiose, but not -mannitol. The principal fatty acids were C, summed feature 3 (iso-C 2-OH and/or Cω) and summed feature 8 (C 7 and/or C 6). The respiratory quinone was Q-9. Polar lipids of strain MC042 comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified glycolipids, an unidentified lipid, an unknown glycolipid and aminolipid. Based on its phenotypic, chemotaxonomic and phylogenetic features, strain MC042 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is MC042 (=KCTC 72033=MCCC 1K03575).

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Keyword(s): farmed Murray cod , head ulcers , polyphasic taxonomy and Pseudomonas piscis sp.nov
© 2020 The Authors
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2020-03-26
2024-04-25
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Pseudomonas piscis sp. nov., isolated from the profound head ulcers of farmed Murray cod (Maccullochella peelii peelii)
Int J Syst Evol Microbiol 70, 2732 (2020); https://doi.org/10.1099/ijsem.0.004101
/content/journal/ijsem/10.1099/ijsem.0.004101
/content/journal/ijsem/10.1099/ijsem.0.004101
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