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Volume 70, Issue 4

sp. nov., sp. nov. and sp. nov., isolated from a subtropical stream in China Free

Abstract

Four strains assigned the names FT13W, FT14W, FT58W and FT68W were isolated from a subtropical stream in PR China. All the strains were Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile with flagella. Comparisons based on 16S rRNA gene sequences showed that strains FT13W, FT14W, FT58W and FT68W belonged to genus and shared 16S rRNA gene similarities in the range of 98.8–99.7 % with DSM 1522, DSM 9628 and ‘ JA-1', respectively. The calculated pairwise average nucleotide identity (ANI) values among the genomes of above seven strains were in the range of 79.0–92.2 %, except that the ANI value was 96.8 % between strain FT13W and FT14W. The respiratory quinone of strains FT13W, FT14W, FT58W and FT68W was determined to be Q-8. The major fatty acids were C ω7, C, C ω7 and C. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The genome sizes of strains FT13W, FT14W, FT58W and FT68W were 6.45, 6.38, 5.73 and 6.37 Mbp with G+C contents of 63.4, 63.7, 61.6 and 63.1 mol%, respectively. Combining phenotypic, biochemical, genotypic and ANI data, strain FT13W and FT14W should belong to the same species. The four strains were considered to represent three novel species within genus , for which the names sp. nov. (type strain FT13W=GDMCC 1.1638=KACC 21319), sp. nov. (FT58W=GDMCC 1.1676=KACC 21468) and sp. nov. (FT68W=GDMCC 1.1677=KACC 21469) are proposed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Janthinobacterium , polyphasic taxonomy and subtropical stream
Funding
This study was supported by the:
  • Guangdong MEPP Fund (Award NO. GDOE(2019)A34)
    • Principle Award Recipient: Meiying Xu
  • GDAS’ Special Project of Science and Technology Development (Award 2019GDASYL-0301002)
    • Principle Award Recipient: Meiying Xu
  • Guangdong Provincial Programs for Science and Technology Development (Award 2019B110205004; 2018B020205003; 2018B030324002)
    • Principle Award Recipient: Meiying Xu
  • National Natural Science Foundation of China (Award 91851202, 51678163)
© 2020 The Authors
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2020-03-16
2024-04-19
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Janthinobacterium violaceinigrum sp. nov., Janthinobacterium aquaticum sp. nov. and Janthinobacterium rivuli sp. nov., isolated from a subtropical stream in China
Int J Syst Evol Microbiol 70, 2719 (2020); https://doi.org/10.1099/ijsem.0.004097
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