sp. nov., isolated from commercial solar salt Free

Abstract

Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848 and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation. Growth of both yellow-coloured strains occurred in the presence of 1–9 % NaCl (w/v; optimum, 2–3 %), at 15–45 °C (optimum, 37 °C) and pH 6–9 (optimum, pH 7). The major fatty acids were iso-C, iso-C and anteiso-C. The cell-wall peptidoglycan was -diaminopimelic acid. The only respiratory quinone was menaquinone-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids. DNA G+C content was 37.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848 and HME7618 clustered with YIM 93174. Strains HMF5848 showed the highest 16S rRNA gene sequence similarities to LMG 22167 (96.1 %), CVS-8 (96.0 %) and YIM 93174 (96.0 %). The values of DNA–DNA hybridization and average nucleotide identity between strains HMF5848 and DSM 16318 were 25.8 and 69.7 %, respectively. On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848 represents a novel species, sp. nov. The type strain is HMF5848 (=KCTC 43010=CECT 9695).

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2020-03-26
2024-03-29
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