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Abstract

Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848 and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation. Growth of both yellow-coloured strains occurred in the presence of 1–9 % NaCl (w/v; optimum, 2–3 %), at 15–45 °C (optimum, 37 °C) and pH 6–9 (optimum, pH 7). The major fatty acids were iso-C, iso-C and anteiso-C. The cell-wall peptidoglycan was -diaminopimelic acid. The only respiratory quinone was menaquinone-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids. DNA G+C content was 37.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848 and HME7618 clustered with YIM 93174. Strains HMF5848 showed the highest 16S rRNA gene sequence similarities to LMG 22167 (96.1 %), CVS-8 (96.0 %) and YIM 93174 (96.0 %). The values of DNA–DNA hybridization and average nucleotide identity between strains HMF5848 and DSM 16318 were 25.8 and 69.7 %, respectively. On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848 represents a novel species, sp. nov. The type strain is HMF5848 (=KCTC 43010=CECT 9695).

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2020-03-26
2024-12-02
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References

  1. Cohn F. Untersuchungen über Bakterien. Beitr Biol Pflanz 1872; 1:127–224
    [Google Scholar]
  2. Logan NA, Bacillus DVP. Bergey's Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc., in association with Bergey's Manual Trust; 2015 pp 1–164
    [Google Scholar]
  3. Shivaji S, Chaturvedi P, Suresh K, Reddy GSN, Dutt CBS et al. Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. Int J Syst Evol Microbiol 2006; 56:1465–1473 [View Article]
    [Google Scholar]
  4. Lee J-C, Lee GS, Park D-J, Kim C-J. Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil. Int J Syst Evol Microbiol 2008; 58:2629–2634 [View Article]
    [Google Scholar]
  5. Liu Y, Du J, Lai Q, Zeng R, Ye D et al. Proposal of nine novel species of the Bacillus cereus group. Int J Syst Evol Microbiol 2017; 67:2499–2508 [View Article]
    [Google Scholar]
  6. Reva ON, Smirnov VV, Pettersson B, Priest FG. Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.). Int J Syst Evol Microbiol 2002; 52:101–107 [View Article]
    [Google Scholar]
  7. Lai Q, Liu Y, Shao Z. Bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (Mugil cephalus). Antonie Van Leeuwenhoek 2014; 105:99–107 [View Article]
    [Google Scholar]
  8. Song L, Liu H, Wang J, Huang Y, Dai X et al. Bacillus oceani sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66:796–800 [View Article]
    [Google Scholar]
  9. Guo L-Y, Ling S-K, Li C-M, Chen G-J, Du Z-J. Bacillus marinisedimentorum sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2018; 68:198–203 [View Article]
    [Google Scholar]
  10. Chen Y-G, Zhang Y-Q, Wang Y-X, Liu Z-X, Klenk H-P et al. Bacillus neizhouensis sp. nov., a halophilic marine bacterium isolated from a sea anemone. Int J Syst Evol Microbiol 2009; 59:3035–3039 [View Article]
    [Google Scholar]
  11. Gugliandolo C, Maugeri TL, Caccamo D, Stackebrandt E. Bacillus aeolius sp. nov. a novel thermophilic, halophilic marine Bacillus species from Eolian Islands (Italy). Syst Appl Microbiol 2003; 26:172–176 [View Article]
    [Google Scholar]
  12. Kumar RM, Kaur G, Kumar A, Bala M, Singh NK et al. Taxonomic description and genome sequence of Bacillus campisalis sp. nov., a member of the genus Bacillus isolated from a solar saltern. Int J Syst Evol Microbiol 2015; 65:3235–3240 [View Article]
    [Google Scholar]
  13. Lim J-M, Jeon CO, Kim C-J. Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 2006; 56:2903–2908 [View Article]
    [Google Scholar]
  14. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp 125–175
    [Google Scholar]
  15. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
    [Google Scholar]
  16. Yoon S-H, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 2017; 110:1281–1286 [View Article]
    [Google Scholar]
  17. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
    [Google Scholar]
  18. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28:1823–1829 [View Article]
    [Google Scholar]
  19. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
    [Google Scholar]
  20. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article]
    [Google Scholar]
  22. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  23. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on systematic bacteriology. Report of the AD hoc Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Bacteriol 1987; 37:463–464
    [Google Scholar]
  24. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  25. Brown AE. Benson’s Microbiological Application Laboratory Manual in General Microbiology, 10th ed. New York: McGraw-Hill; 2007
    [Google Scholar]
  26. CLSI Performance Standards for Antimicrobial Disk Susceptibility Testing: Approved Standard. CLSI document M02-A11, 11th ed. PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  27. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  28. Minnikin DE, Alshamaony L, Goodfellow M. Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 1975; 88:200–204 [View Article]
    [Google Scholar]
  29. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  30. Collins MD. Analysis of isoprenoid quinones. In Gottschalk G. editor Methods in Microbiology 18 New York: Acad. Press; 1985 pp 329–366
    [Google Scholar]
  31. Shi R, Yin M, Tang S-K, Lee J-C, Park D-J et al. Bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field. Int J Syst Evol Microbiol 2011; 61:1344–1349 [View Article]
    [Google Scholar]
  32. Kosowski K, Schmidt M, Pukall R, Hause G, Kämpfer P et al. Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor. Int J Syst Evol Microbiol 2014; 64:88–94 [View Article]
    [Google Scholar]
  33. Heyrman J, Rodríguez-Díaz M, Devos J, Felske A, Logan NA et al. Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 2005; 55:111–117 [View Article]
    [Google Scholar]
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