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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

gen. nov., sp. nov., a novel member of the family isolated from an activated sludge sample Free

Abstract

A coccobacilli-shaped proteobacterium, designated strain SYSU XM001, was isolated from an activated sludge sample collected from JiMei sewage treatment plant, Xiamen, PR China. Cells were Gram-stain-negative, catalase-positive and oxidase-negative. The 16S rRNA gene sequence of strain SYSU XM001 shared less than 92 % sequence identities with members of the class , with highest sequence similarity to TH1-2 (91.6 %; family , order ). The strain exhibited growth at 25–37 °C, pH 7.0–9.0 and in the presence of up to 1 % (w/v) NaCl. Its chemotaxonomic features included ubiquinone-10 as the respiratory isoprenologue, iso-C, 10-methyl C TSBA and anteiso-C as major cellular fatty acids and monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids as the main polar lipids. The DNA G+C content was determined to be 62.9 % (draft genome). Analyses of the phylogenetic data and differences in the chemotaxonomic and biochemical features from related genera in the family indicated that strain SYSU XM001 merits representation of a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is SYSU XM001 (=CGMCC 1.16661=KCTC 62915).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): activated sludge , Hyphomonadaceae and Vitreimonas flagellata gen. nov., sp. nov
Funding
This study was supported by the:
  • Natural Science Foundation of Guangdong Province (Award 2016A030312003)
    • Principle Award Recipient: Wen-Jun Li
  • National Natural Science Foundation of China (Award 31500100)
    • Principle Award Recipient: Wen-Jun Li
  • National Natural Science Foundation of China (Award 31850410475)
    • Principle Award Recipient: Nimaichand Salam
  • National Natural Science Foundation of China (Award 31670492)
    • Principle Award Recipient: Feng Guo
© 2020 Sun Yat-Sen University, PR China
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2020-03-05
2023-12-04
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Vitreimonas flagellata gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from an activated sludge sample
Int J Syst Evol Microbiol 70, 2632 (2020); https://doi.org/10.1099/ijsem.0.004082
/content/journal/ijsem/10.1099/ijsem.0.004082
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