1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov. and sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae Free

Abstract

Seven yeast strains, DMKU VGT1-14, DMKU VGT1-19, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DMKU VGT2-19 and DMKU VGT6-14, were isolated from a grease trap in Thailand and two strains, SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, these strains represented two novel yeast species of the genus . In terms of pairwise sequence similarity, four strains, DMKU VGT1-14, DMKU-JMGT1-32, DMKU VGT6-14 and SN-102, were closely related to NRRL Y-17858 but differed by 13 nucleotide substitutions with one gap (2.46 %) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2 %) in the ITS region. The strains DMKU VGT1-19, DMKU-JMGT1-28, DMKU VGT2-06, DMKU VGT2-19 and SJ-1, differed from the type strain of the most closely related species, NRRL Y-7921, by nine nucleotide substitutions with one gap (1.66 %) in the D1/D2 domain of the LSU rRNA gene and nine nucleotide substitutions with 17 gaps (2.52%) in the ITS region. Hence, the names sp. nov. and sp. nov. are proposed to accommodate these species in the genus . The holotypes are DMKU VGT1-14 (ex-type strain TBRC 11425=PYCC 8359=CGMCC 2.6179; Mycobank number 833394) and DMKU VGT1-19 (ex-type strain TBRC 11426=PYCC 8360=CGMCC 2.6180; Mycobank number 833393).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ascomycetous yeast , grease trap , novel yeast species and osmotolerant yeast
Funding
This study was supported by the:
  • Thailand Research Fund (Award PHD/0070/2560)
    • Principle Award Recipient: Varunya Sakpuntoon
  • Thailand Research Fund (Award RTA608004)
    • Principle Award Recipient: Savitree Limtong
© 2020 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: sp. nov. and sp. nov., novel ascomycetous yeast in the family Trichomonascaceae
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004075
2020-03-05
2023-11-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/4/2596.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004075&mimeType=html&fmt=ahah

References

  1. van der Walt JP, Liebenberg NV. The yeast genus Wickerhamiella gen. nov. (Ascomycetes). Antonie Van Leeuwenhoek 1973; 39:121–128 [View Article]
     [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov. Antonie van Leeuwenhoek 2014; 105:933–942 [View Article]
     [Google Scholar]
  3. Avchar R, Groenewald M, Baghela A. Wickerhamiella shivajii sp. nov., a thermotolerant yeast isolated from distillery effluent. Int J Syst Evol Microbiol 2019; 69:3262–3267 [View Article]
     [Google Scholar]
  4. Khunnamwong P, Surussawadee J, Jindamorakot S, Limtong S. Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf. Int J Syst Evol Microbiol 2014; 64:3849–3855 [View Article]
     [Google Scholar]
  5. Badotti F, Silva PAB, Mendonça MC, Gomes FCO, Morais PB et al. Wickerhamiella dulcicola sp. nov. and Wickerhamiella cachassae sp. nov., yeasts isolated from cachaça fermentation in Brazil. Int J Syst Evol Microbiol 2013; 63:1169–1173 [View Article]
     [Google Scholar]
  6. Hagler AN, Ribeiro JRA, Pinotti T, Brandão LR, Pimenta RS et al. Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates. Int J Syst Evol Microbiol 2013; 63:3099–3103 [View Article]
     [Google Scholar]
  7. Chang C-F, Lee C-F, Liu S-M. Cryptococcus keelungensis sp. nov., an anamorphic basidiomycetous yeast isolated from the sea-surface microlayer of the north-east coast of Taiwan. Int J Syst Evol Microbiol 2008; 58:2973–2976 [View Article]
     [Google Scholar]
  8. Lachance M, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida Berkhout the yeasts, a taxonomic study; 1923987–1278
  9. Kurtzman CP, Robnett CJ. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 2003; 3:417–432 [View Article]
     [Google Scholar]
  10. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser 1999; 41:95–98
     [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  12. Kurtzman C, Fell J, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman C, Fell J, Boekhout T. (editors) The Yeast, a Taxonomic Study. 1, 5 ed. San Diego: Elsevier; 2011 pp 105–106
     [Google Scholar]
  13. Fowell RR. Sodium acetate agar as a sporulation medium for yeast. Nature 1952; 170:578 [View Article]
     [Google Scholar]
  14. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 1998; 73:331–371 [View Article]
     [Google Scholar]
  15. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article]
     [Google Scholar]
  16. Kurtzman CP. New anamorphic yeast species: Candida infanticola sp. nov., Candida polysorbophila sp. nov., Candida transvaalensis sp. nov. and Trigonopsis californica sp. nov. Antonie Van Leeuwenhoek 2007; 92:221–231 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004075
Loading
Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae
Int J Syst Evol Microbiol 70, 2596 (2020); https://doi.org/10.1099/ijsem.0.004075
/content/journal/ijsem/10.1099/ijsem.0.004075
/content/journal/ijsem/10.1099/ijsem.0.004075
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error