1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov., isolated from human semen Free

Abstract

Two -like isolates with similar biochemical characteristics, designated strains SZY H1 and SZY H2, were isolated from human semen specimens. Cells were Gram-negative, non-motile, non-acid-fast, pleomorphic rods or coccobacilli. The major fatty acids (>10 %) were C, C, iso-C and/or C 3-OH and C 6 and/or C 7. The polar lipids were determined to be phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminophospholipid, two unidentified polar lipids and four unidentified aminolipids. The major polyamine was found to be cadaverine. The near-full-length (1462 nt) 16S rRNA gene sequences analysis showed the two isolates were nearly identical (>99.8 %), and closely matched ATCC 33390 with 98.9–99.1 % sequence similarities. Phylogenetic analysis based on 16S rRNA gene sequences and concatenation of 30 protein markers also revealed that the isolates clustered together with ATCC 33390, and formed a distinct lineage well separated from the other members of the genus . Further, the average nucleotide identity values between the two isolates and their related species were below the established cut-off values for species delineation (95 %). Based on these findings, the two isolates are considered to represent a new species of the genus , for which name sp. nov. is proposed. The type strain is SZY H1 (=NBRC 113782=CGMCC 1.17137).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Haemophilus seminalis sp. nov , human semen and polyphasic taxonomy
Funding
This study was supported by the:
  • Research Fund for Science and Technology Planning Project of Guangdong Province, China
© 2020 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004074
2020-03-12
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/4/2588.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004074&mimeType=html&fmt=ahah

References

  1. Kilian M. Genus III. Haemophilus. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, second edition, vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria) New York: Springer; 2005 pp 883–885
     [Google Scholar]
  2. Dewhirst FE, Paster BJ, Olsen I, Fraser GJ. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 1992; 174:2002–2013 [View Article]
     [Google Scholar]
  3. Nørskov-Lauritsen N, Kilian M. Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol 2006; 56:2135–2146 [View Article]
     [Google Scholar]
  4. Blackall PJ, Christensen H, Beckenham T, Blackall LL, Bisgaard M. Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov. Int J Syst Evol Microbiol 2005; 55:353–362 [View Article]
     [Google Scholar]
  5. Nørskov-Lauritsen N. Classification, identification, and clinical significance of Haemophilus and Aggregatibacter species with host specificity for humans. Clin Microbiol Rev 2014; 27:214–240 [View Article]
     [Google Scholar]
  6. Lo CI, Sankar SA, Fall B, Sambe-Ba B, Diawara S et al. High-quality draft genome sequence and description of Haemophilus massiliensis sp. nov. Stand Genomic Sci 2016; 11:31 [View Article]
     [Google Scholar]
  7. Albritton WL, Brunton JL, Meier M, Bowman MN, Slaney LA. Haemophilus influenzae: comparison of respiratory tract isolates with genitourinary tract isolates. J Clin Microbiol 1982; 16:826–831 [View Article]
     [Google Scholar]
  8. Quentin R, Musser JM, Mellouet M, Sizaret PY, Selander RK et al. Typing of urogenital, maternal, and neonatal isolates of Haemophilus influenzae and Haemophilus parainfluenzae in correlation with clinical source of isolation and evidence for a genital specificity of H. influenzae biotype IV. J Clin Microbiol 1989; 27:2286–2294 [View Article]
     [Google Scholar]
  9. Quentin R, Goudeau A, Wallace RJ, Smith AL, Selander RK et al. Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species. J Gen Microbiol 1990; 136:1203–1209 [View Article]
     [Google Scholar]
  10. Cardines R, Daprai L, Giufrè M, Torresani E, Garlaschi ML et al. Genital carriage of the genus Haemophilus in pregnancy: species distribution and antibiotic susceptibility. J Med Microbiol 2015; 64:724–730 [View Article]
     [Google Scholar]
  11. Deza G, Martin-Ezquerra G, Gómez J, Villar-García J, Supervia A et al. Isolation of Haemophilus influenzae and Haemophilus parainfluenzae in urethral exudates from men with acute urethritis: a descriptive study of 52 cases. Sex Transm Infect 2016; 92:29–31 [View Article]
     [Google Scholar]
  12. Glover WA, Suarez CJ, Clarridge JE. Genotypic and phenotypic characterization and clinical significance of 'Haemophilus quentini' isolated from the urinary tract of adult men. J Med Microbiol 2011; 60:1689–1692 [View Article]
     [Google Scholar]
  13. Kus JV, Shuel M, Soares D, Hoang W, Law D et al. Identification and characterization of "Haemophilus quentini" Strains Causing Invasive Disease in Ontario, Canada (2016 to 2018). J Clin Microbiol 2019; 57: [View Article]
     [Google Scholar]
  14. Christensen H, Kuhnert P, Busse H-J, Frederiksen WC, Bisgaard M. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae . Int J Syst Evol Microbiol 2007; 57:166–178 [View Article]
     [Google Scholar]
  15. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article]
     [Google Scholar]
  16. Krishna M, Gole SG. Comparison of conventional Ziehl-Neelsen method of acid fast bacilli with modified bleach method in tuberculous lymphadenitis. J Cytol 2017; 34:188–189 [View Article]
     [Google Scholar]
  17. Ming H, Nie G-X, Jiang H-C, Yu T-T, Zhou E-M et al. Paenibacillus frigoriresistens sp. nov., a novel psychrotroph isolated from a peat bog in Heilongjiang, Northern China. Antonie Van Leeuwenhoek 2012; 102:297–305 [View Article]
     [Google Scholar]
  18. Møller K, Kilian M. V factor-dependent members of the family Pasteurellaceae in the porcine upper respiratory tract. J Clin Microbiol 1990; 28:2711–2716 [View Article]
     [Google Scholar]
  19. Inzana TJ, Johnson JL, Shell L, Møller K, Kilian M. Isolation and characterization of a newly identified Haemophilus species from cats: "Haemophilus felis". J Clin Microbiol 1992; 30:2108–2112 [View Article]
     [Google Scholar]
  20. Kilian M. A rapid method for the differentiation of Haemophilus strains. The porphyrin test;. Acta Pathol Microbiol Scand B Microbiol Immunol 1974; 82:835–842 [View Article]
     [Google Scholar]
  21. Hammond GW, Lian CJ, Wilt JC, Albritton WL, Ronald AR. Determination of the hemin requirement of Haemophilus ducreyi: evaluation of the porphyrin test and media used in the satellite growth test. J Clin Microbiol 1978; 7:243–246
     [Google Scholar]
  22. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol 1976; 93:9–62 [View Article]
     [Google Scholar]
  23. Aslanzadeh J. Biochemical profile-based microbial identification systems [M]. In Tang YW, Stratton CW. (editors) Advanced techniques in diagnostic microbiology New York: Springer; 2006 pp 87–121
     [Google Scholar]
  24. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 1990; 20:16–17
     [Google Scholar]
  25. Scherer P, Kneifel H. Distribution of polyamines in methanogenic bacteria. J Bacteriol 1983; 154:1315–1322 [View Article]
     [Google Scholar]
  26. Busse H-J, Bunka S, Hensel A, Lubitz W. Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Evol Microbiol 1997; 47:698–708 [View Article]
     [Google Scholar]
  27. Minnikin DE, Collins MD, Goodfellow M. Fatty Acid and Polar Lipid Composition in the Classification of Cellulomonas, Oerskovia and Related Taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
     [Google Scholar]
  28. He Y, Chang TC, Li H, Shi G, Tang Y-W. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database for identification of Legionella species. Can J Microbiol 2011; 57:533–538 [View Article]
     [Google Scholar]
  29. Li W-J, Xu P, Schumann P, Zhang Y-Q, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article]
     [Google Scholar]
  30. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
     [Google Scholar]
  31. Edgar RC. Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article]
     [Google Scholar]
  32. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article]
     [Google Scholar]
  33. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
     [Google Scholar]
  34. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  35. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  36. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  37. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1984
     [Google Scholar]
  38. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  39. Harrison P, Strulo B. SPADES - a process algebra for discrete event simulation. Journal of Logic and Computation 2000; 10:3–42 [View Article]
     [Google Scholar]
  40. Hyatt D, Chen G-L, Locascio PF, Land ML, Larimer FW et al. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics 2010; 11:119 [View Article]
     [Google Scholar]
  41. Wu M, Scott AJ. Phylogenomic analysis of bacterial and archaeal sequences with AMPHORA2. Bioinformatics 2012; 28:1033–1034 [View Article]
     [Google Scholar]
  42. Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 2000; 17:540–552 [View Article]
     [Google Scholar]
  43. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article]
     [Google Scholar]
  44. Letunic I, Bork P. Interactive tree of life (iTOL) V4: recent updates and new developments. Nucleic Acids Res 2019; 47:W256W259 [View Article]
     [Google Scholar]
  45. Meats E, Feil EJ, Stringer S, Cody AJ, Goldstein R et al. Characterization of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. J Clin Microbiol 2003; 41:1623–1636 [View Article]
     [Google Scholar]
  46. Nørskov-Lauritsen N, Overballe MD, Kilian M. Delineation of the species Haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes. J Bacteriol 2009; 191:822–831 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004074
Loading
Haemophilus seminalis sp. nov., isolated from human semen
Int J Syst Evol Microbiol 70, 2588 (2020); https://doi.org/10.1099/ijsem.0.004074
/content/journal/ijsem/10.1099/ijsem.0.004074
/content/journal/ijsem/10.1099/ijsem.0.004074
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error