RT Journal Article SR Electronic(1) A1 Fu, Tianyu A1 Cui, Hongchang A1 Jia, Chao A1 Sun, Wen A1 Zhou, Shun A1 Han, Yanqiong A1 Liu, Lijun A1 Shi, Xiaochong A1 Zhang, Xiao-HuaYR 2020 T1 Ancylomarina longa sp. nov., isolated from southern Okinawa Trough sediment and emended description of the family Marinifilaceae JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 4 SP 2531 OP 2536 DO https://doi.org/10.1099/ijsem.0.004066 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, obligately anaerobic, non-motile, non-spore-forming, long-rod-shaped and non-flagellated bacterial strain, designated T3-2 S1-CT, was isolated from a sediment sample collected at the Okinawa Trough. Phylogenetic analyses of 16S rRNA gene sequences and the whole genome revealed that strain T3-2 S1-CT was a member of the family Marinifilaceae and exhibited less than 95.1 % sequence similarities to the closely related type strains of the family Marinifilaceae . Optimal growth occurred at pH 7.0, 28 °C and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain T3-2 S1-CT was identified as menaquinone-7 (MK-7) and the predominant fatty acids (>10 %) were iso-C15 : 0 (38.9 %) and anteiso-C15 : 0 (11.6 %). The major polar lipids were one phosphatidylethanolamine, one phosphatidylmonomethylethanolamine, one aminolipids, two unidentified lipids and two unidentified phospholipids. The DNA G+C content of strain T3-2 S1-CT was 35.7 mol%. On the basis of the results of polyphasic analyses, strain T3-2 S1-CT is considered to represent a novel species of the genus Ancylomarina , for which the name Ancylomarina longa sp. nov. is proposed. The type strain is T3-2 S1-CT (=KCTC 15505T=MCCC 1K01617T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004066