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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov., isolated from southern Okinawa Trough sediment and emended description of the family Free

Abstract

A Gram-stain-negative, obligately anaerobic, non-motile, non-spore-forming, long-rod-shaped and non-flagellated bacterial strain, designated T3-2 S1-C, was isolated from a sediment sample collected at the Okinawa Trough. Phylogenetic analyses of 16S rRNA gene sequences and the whole genome revealed that strain T3-2 S1-C was a member of the family and exhibited less than 95.1 % sequence similarities to the closely related type strains of the family . Optimal growth occurred at pH 7.0, 28 °C and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain T3-2 S1-C was identified as menaquinone-7 (MK-7) and the predominant fatty acids (>10 %) were iso-C (38.9 %) and anteiso-C (11.6 %). The major polar lipids were one phosphatidylethanolamine, one phosphatidylmonomethylethanolamine, one aminolipids, two unidentified lipids and two unidentified phospholipids. The DNA G+C content of strain T3-2 S1-C was 35.7 mol%. On the basis of the results of polyphasic analyses, strain T3-2 S1-C is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T3-2 S1-C (=KCTC 15505=MCCC 1K01617).

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Aancylomarina longa , Okinawa and sediment
Funding
This study was supported by the:
  • the National Key R&D Program of China (Award 2017YFA0603200)
    • Principle Award Recipient: Xiaochong Shi
  • China Ocean Mineral Resources R & D Association (Award DY135-B2-10)
    • Principle Award Recipient: Xiaochong Shi
  • Research and Development Program for the South China Sea of the Sansha Trackline Ocean Coral Reef Conservation Research Institute (Award HJLD-1701)
    • Principle Award Recipient: Xiaochong Shi
  • OUC-AU joint projects
    • Principle Award Recipient: Xiaochong Shi
© 2020 The Authors
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2020-02-20
2024-04-23
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Ancylomarina longa sp. nov., isolated from southern Okinawa Trough sediment and emended description of the family Marinifilaceae
Int J Syst Evol Microbiol 70, 2531 (2020); https://doi.org/10.1099/ijsem.0.004066
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