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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 4

sp. nov., isolated from cool temperate forest soil Free

Abstract

A Gram-stain-negative, aerobic and short rod-shaped bacterial strain, designated LD6, was isolated from a forest soil sample in Suwon, Gyeonggi-do, Republic of Korea. Strain LD6 grew at 10–37 °C (optimal temperature, 28 °C), and tolerated pH 8.0 and 2 % (w/v) NaCl. Strain LD6 was related most closely to members of the genus , namely NF2-5-3 (98.2 % 16S rRNA gene sequence similarity), A3 (97.9 %), DCY85 (97.9 %) and MWAP64 (97.7 %). The strain grew well on R2A agar, tryptone soya agar, Mueller-Hinton agar and nutrient agar. The major polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and glycolipid. The major respiratory quinone was ubiquinone 8 (Q-8). The main fatty acids were C cyclo, C, C 3-OH, C cyclo ω8 and C. The DNA G+C content of the isolated strain based on the whole genome sequence was 63.4 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain LD6 and its reference type strains ranged from 80.3 to 82.4%, and from 23.7 to 33.7%, respectively. Based on phenotypic, chemotypic and genotypic evidence, strain LD6 could be differentiated phylogenetically and phenotypically from the recognized species of the genus . Therefore, strain LD6 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is LD6 (=KACC 21387=JCM 33640).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): antibiotics , cool temperate forest soil , novel species , Paraburkholderia flava , Proteobacteria and Suwon
Funding
This study was supported by the:
  • Kyonggi University (Award 2018-031)
    • Principle Award Recipient: Jaisoo Kim
© 2020 The Authors
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2020-02-26
2025-03-20
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Paraburkholderia flava sp. nov., isolated from cool temperate forest soil
Int J Syst Evol Microbiol 70, 2509 (2020); https://doi.org/10.1099/ijsem.0.004063
/content/journal/ijsem/10.1099/ijsem.0.004063
/content/journal/ijsem/10.1099/ijsem.0.004063
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