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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov., isolated from traditional Chinese yogurt Free

Abstract

Three Gram-stain-positive bacterial strains, designated X0750, X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 16S rRNA gene and three housekeeping genes (, and ), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), DNA–DNA hybridization (DDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750 was phylogenetically related to the type strains of , , , , , , and , having 94.4–100 % 16S rRNA gene sequence similarities, 76.7–90.0 %  gene sequence similarities, 88.9–99.4 %  gene sequence similarities and 77.6–92.8 %  gene sequence similarities, respectively. ANI, DDH and AAI values between strain X0750 and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8 % respectively, confirming that strain X0750 represents a novel species within the genus . Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is X0750(=NCIMB 15192=CCM 8924=LMG 31184=CCTCC AB 2018403).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): lactic acid bacteria , novel species , Weissella and yogurt
Funding
This study was supported by the:
  • the Agricultural Science and Technology Innovation Program, China (ASTIP) and the Building of Modern Agricultural Industry (Bees) R&D Systems in China (Award NYCYTI-43-KXJ17)
    • Principle Award Recipient: Wen Li Tian
  • National Natural Science Foundation of China (Award 31972087)
    • Principle Award Recipient: Wen Li Tian
  • National Natural Science Foundation of China (Award 31471594)
    • Principle Award Recipient: Chun Tao Gu
© 2020 The Authors
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2020-02-25
2024-04-24
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Weissella sagaensis sp. nov., isolated from traditional Chinese yogurt
Int J Syst Evol Microbiol 70, 2485 (2020); https://doi.org/10.1099/ijsem.0.004062
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