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Volume 70, Issue 4

sp. nov., isolated from snow finch () cloacal content Free

Abstract

A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai–Tibet Plateau, PR China. Analysis of the 16S rRNA gene sequence showed that strain CF-49 was closely related to CCUG 51432 (96.5 % similarity), NCFB 2497 (96.0 %) and CCUG 39187 (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %. Strains CF-49 and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl. Acid was produced from -acetylglucosamine, cellobiose, -fructose, -glucose, -mannose, -mannitol, maltose, -ribose and salicin. The cell-wall peptidoglycan type was A4α (-Lys-Asp). The major cellular fatty acids (>10 %) were C (35.6 %), C (17.3 %), C ω9 (16.2 %) and C ω9 (10.6 %). The predominant respiratory quinone was menaquinone MK-7 (68.8 %). The G+C content of the genomic DNA was 35.9 mol%. Digital DNA–DNA hybridization of strain CF-49 with DSM 5731, CCUG 51432and CCUG 39187 resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively. Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus , with the name . The type strain is CF-49 (=CGMCC 1.6436=GDMCC 1.1588=JCM 33477).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): novel species , Qinghai-Tibet Plateau , snow finch and Vagococcus
Funding
This study was supported by the:
  • Chinese Academy of Medical Sciences and Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: Jianguo Xu
  • National Science and Technology Major Project of China (Award 2018ZX10305409-003)
    • Principle Award Recipient: Zhihong Ren
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
© 2020 The Authors
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2020-03-20
2024-04-19
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Vagococcus xieshaowenii sp. nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content
Int J Syst Evol Microbiol 70, 2493 (2020); https://doi.org/10.1099/ijsem.0.004061
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