1887

Abstract

An anaerobic and aerotolerant bacterium, strain M12, was isolated from the meibum of inflamed human meibomian glands. Cells of the strain was Gram-stain-positive, non-spore-forming and non-motile rods. Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30–37 °C under anaerobic conditions. The 16S rRNA gene sequence of the strain revealed that it belongs to the genus with a 98.0 % similarity value to the closest species, . Genome analysis of the strain with type strains of the other species resulted in digital DNA–DNA hybridization values of 32.3–22.3% and average nucleotide identity (OrthoANI) values of 86.7–73.6 %. Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with and . The most abundant major cellular fatty acid was iso-C. Fermentation end-products from glucose were propionate, lactate, succinate and acetate. The diagnostic diamino acid of the peptidoglycan was diaminopimelic acid. Major menaquinones were MK-9(H), MK-9(H) and MK-9. The major peaks of the MALDI-TOF mass spectrometry spectrum were at 3493, 3712, 6986 and 7424 Da. The DNA G+C content was 59.9 mol%. Based on these findings, we propose a novel species, . The type strain of is M12 (=JCM 33380=DSM 109769). On the basis of further genomic analysis, we also provide emended descriptions of (Prévot 1938) Scholz and Kilian 2016 and (Aubin . 2016) Nouioui 2018.

Funding
This study was supported by the:
  • Tomo Suzuki , Japan Society for the Promotion of Science , (Award 16K11295)
  • Mitsuo Sakamoto , Japan Agency for Medical Research and Development , (Award JP19gm6010007)
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2020-02-19
2020-06-02
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