1887

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Volume 70, Issue 4

sp. nov., a marine, cellulolytic endosymbiotic bacterium isolated from the gills of the wood-boring mollusc (Bivalvia: Teredinidae) and emended description of the genus Open Access

Abstract

A cellulolytic, aerobic, gammaproteobacterium, designated strain Bs02, was isolated from the gills of a marine wood-boring mollusc, (Bivalvia: Teredinidae). The cells are Gram-stain-negative, slightly curved motile rods (2–5×0.4–0.6 µm) that bear a single polar flagellum and are capable of heterotrophic growth in a simple mineral medium supplemented with cellulose as a sole source of carbon and energy. Cellulose, carboxymethylcellulose, xylan, cellobiose and a variety of sugars also support growth. Strain Bs02 requires combined nitrogen for growth. Temperature, pH and salinity optima (range) for growth were 20 °C (range, 10–30 °C), 8.0 (pH 6.5–8.5) and 0.5 M NaCl (range, 0.0–0.8 M), respectively when grown on 0.5 % (w/v) galactose. Strain Bs02 does not require magnesium and calcium ion concentrations reflecting the proportions found in seawater. The genome size is approximately 4.03 Mbp and the DNA G+C content of the genome is 47.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences, and on conserved protein-coding sequences, show that strain Bs02 forms a well-supported clade with . Average nucleotide identity and percentage of conserved proteins differentiate strain Bs02 from at threshold values exceeding those proposed to distinguish bacterial species but not genera. These results indicate that strain Bs02 represents a novel species in the previously monotypic genus for which the name sp. nov. is proposed. The strain has been deposited under accession numbers ATCC TSD-120 and KCTC 62963.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Bankia setacea , bivalve , cellulolytic symbiont , shipworm , Teredinibacter and Teredinidae
Funding
This study was supported by the:
  • NOAA Research (Award NA19OAR010303)
    • Principle Award Recipient: Daniel L Distel
  • Directorate for Biological Sciences (Award DBI 1722553)
    • Principle Award Recipient: Not Applicable
  • Directorate for Biological Sciences (Award IOS 1442759)
    • Principle Award Recipient: Daniel L Distel
  • Fogarty International Center (Award U19TW008163)
    • Principle Award Recipient: Daniel L Distel
  • Fogarty International Center (Award U19TW008163)
    • Principle Award Recipient: Margo G Haygood
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons.
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2020-02-20
2024-04-23
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Teredinibacter waterburyi sp. nov., a marine, cellulolytic endosymbiotic bacterium isolated from the gills of the wood-boring mollusc Bankia setacea (Bivalvia: Teredinidae) and emended description of the genus Teredinibacter
Int J Syst Evol Microbiol 70, 2388 (2020); https://doi.org/10.1099/ijsem.0.004049
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