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Volume 70, Issue 4

sp. nov., a novel bacterium isolated from soil sampled at Tiandong Cave Free

Abstract

A Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain 5.0403-2, was isolated from a cave soil sample collected from Tiandong Cave, Guizhou Province, south-west PR China. Cells showed positive oxidase and catalase reactions. The predominant isoprenoid quinone was MK-7. The major fatty acids were identified as iso-C, summed feature 3 (C ω7 and/or C ω6), iso-C 3OH and summed feature 9 (iso-C ω9 or C 10-methyl). The cellular polar lipids contained phosphatidylethanolamine, one unidentified phospholipid, three unidentified phosphoglycolipids and four unidentified lipids. The genomic DNA G+C content was 36.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5.0403-2 should be assigned to the genus . Results of 16S rRNA gene sequence similarity analysis showed that strain 5.0403-2 was most similar to KCTC 52685 (98.7 %), KCTC 12578 (98.0 %) and DSM 22362 (97.3 %) and less than 95.0 % similar to other species of the genus . The average nucleotide identity values between strain 5.0403-2 and KCTC 52685, KCTC 12578 and DSM 22362 were 94.2, 82.3 and 77.2 % respectively. The digitalDNA–DNA hybridization values between strain 5.0403-2 and KCTC 52685, KCTC 12578 and DSM 22362 were 68.4, 25.6 and 20.7 %. These results indicated that the isolate represented a novel genomic species. The polyphasic taxonomic characteristics indicated that strain 5.0304-2 represents a novel species of the genus , for which the name sp. nov. (type strain 5.0403–2=KCTC 62981=CCTCC AB 2019257) is proposed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): cave soil , polyphasic taxonomy and Sphingobacterium cavernae sp. nov.
Funding
This study was supported by the:
  • Secretaría de Ciencia y Tecnología, Gobierno de la Provincia de Córdoba (AR) (Award 2017FB020)
    • Principle Award Recipient: Ming He Mo
  • China Tobacco Guizhou Industrial (CN) (Award 2018530000241008)
    • Principle Award Recipient: Ming He Mo
  • the projects from National Natural Science Foundation of China (Award 31660023)
    • Principle Award Recipient: Ming He Mo
  • Key Technology Research and Development Program of Shandong (Award No. 2017YFD0201104)
    • Principle Award Recipient: Ming He Mo
  • the Department of Science and Technology of Yunnan Province (Award No. 2018BC001)
    • Principle Award Recipient: Ming He Mo
  • Cultivating Plan Program for the Leader in Science and Technology of Yunnan Province (CN) (Award No. 2017FA016)
    • Principle Award Recipient: Ming He Mo
  • China Tobacco Guizhou Industrial (Award No. 2019530000241018)
    • Principle Award Recipient: Ming He Mo
  • China Tobacco Yunnan Industrial Co. Ltd. (Award No.2018530000241019)
    • Principle Award Recipient: Ming He Mo
  • Innovative Research Group Project of the National Natural Science Foundation of China (CN) (Award No. 31960022)
    • Principle Award Recipient: Ming He Mo
  • the projects from National Natural Science Foundation of China (Award No. 31870091)
    • Principle Award Recipient: Ming He Mo
© 2020 The Authors
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2024-04-19
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Sphingobacterium cavernae sp. nov., a novel bacterium isolated from soil sampled at Tiandong Cave
Int J Syst Evol Microbiol 70, 2348 (2020); https://doi.org/10.1099/ijsem.0.004044
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