Two strains, WS 5063T and WS 5067, isolated from raw cow’s milk and skimmed milk concentrate, could be affiliated as members of the same, hitherto unknown, Pseudomonas species by 16S rRNA and rpoD gene sequences. Multilocus sequence and average nucleotide identity (ANIm) analyses based on draft genome sequences confirmed the discovery of a novel Pseudomonas species. It was most closely related to Pseudomonas synxantha DSM 18928T with an ANIm of 91.4 %. The DNA G+C content of WS 5063T was 60.0 mol %. Phenotypic characterizations showed that the isolates are rod-shaped, motile, catalase- and oxidase-positive, and aerobic. Growth occurred at 4–34 °C and at pH values of pH 5.5–8.0. Both strains showed strong β-haemolysis on blood agar. The major cellular polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The dominant quinone was Q-9 (90 %), but noticeable amounts of Q-8 (9 %) and traces of Q-7 were also detected. Fatty acid profiles were typical for Pseudomonas species and exhibited C16 : 0 as a major component. Based on these results, we conclude that both strains belong to a novel species, for which the name Pseudomonas haemolytica sp. nov. is proposed. The type strain is WS 5063T (=DSM 108987T=LMG 31232T) and an additional strain is WS 5067 (=DSM 108988=LMG 31233).
BallokAE,
O'TooleGA.
Pouring salt on a wound: Pseudomonas aeruginosa virulence factors alter Na+ and Cl- flux in the lung. J Bacteriol2013; 195:4013–4019 [View Article]
Hantsis-ZacharovE,
HalpernM.
Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. Appl Environ Microbiol2007; 73:7162–7168 [View Article]
von NeubeckM,
BaurC,
KrewinkelM,
StoeckelM,
KranzB et al. Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential. Int J Food Microbiol2015; 211:57–65 [View Article]
MarchandS,
HeylenK,
MessensW,
CoudijzerK,
De VosP et al. Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples. Environ Microbiol2009; 11:467–482 [View Article]
de OliveiraGB,
FavarinL,
LucheseRH,
McIntoshD.
Psychrotrophic bacteria in milk: how much do we really know?. Braz J Microbiol2015; 46:313–321 [View Article]
LafargeV,
OgierJ-C,
GirardV,
MaladenV,
LeveauJ-Y et al. Raw cow milk bacterial population shifts attributable to refrigeration. Appl Environ Microbiol2004; 70:5644–5650 [View Article]
VithanageNR,
DissanayakeM,
BolgeG,
PalomboEA,
YeagerTR et al. Biodiversity of culturable psychrotrophic microbiota in RAW milk attributable to refrigeration conditions, seasonality and their spoilage potential. Int Dairy J2016; 57:80–90 [View Article]
MuletM,
BennasarA,
LalucatJ,
García-ValdésE.
An rpoD-based PCR procedure for the identification of Pseudomonas species and for their detection in environmental samples. Mol Cell Probes2009; 23:140–147 [View Article]
ChunJ,
OrenA,
VentosaA,
ChristensenH,
ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2018; 68:461–466 [View Article]
HuptasC,
SchererS,
WenningM.
Optimized illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assembly. BMC Res Notes2016; 9:269 [View Article]
BankevichA,
NurkS,
AntipovD,
GurevichAA,
DvorkinM et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol2012; 19:455–477 [View Article]
ParksDH,
ImelfortM,
SkennertonCT,
HugenholtzP,
TysonGW.
CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res2015; 25:1043–1055 [View Article]
ChainPSG,
GrafhamDV,
FultonRS,
FitzgeraldMG,
HostetlerJ et al. Genomics. genome Project standards in a new era of sequencing. Science2009; 326:236–237 [View Article]
RichterM,
Rosselló-MóraR.
Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A2009; 106:19126–19131 [View Article]
EhrenbergCG.
Charakteristik von 274 neuen Arten von Infusorien. Bericht bekannter Verhandlungen der Königlich-Preussischen Akademie der Wissenschaftlichen Berlin: 1840 pp 197–219
HollandDF.
Generic index of the commoner forms of bacteria. In
Winslow CEAJB,
BuchananRE,
KrumwiedeC,
RogersLA.
(editors) The families and genera of the bacteria Journal of Bacteriology; 1920 pp 191–229
DabboussiF,
HamzeM,
ElomariM,
VerhilleS,
BaidaN et al.Pseudomonas libanensis sp. nov., a new species isolated from Lebanese spring waters. Int J Syst Bacteriol1999; 49:1091–1101 [View Article]
von NeubeckM,
HuptasC,
GlückC,
KrewinkelM,
StoeckelM et al.Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk. Int J Syst Evol Microbiol2017; 67:1656–1664 [View Article]
TambongJT,
XuR,
BromfieldESP.
Pseudomonas canadensis sp. nov., a biological control agent isolated from a field plot under long-term mineral fertilization. Int J Syst Evol Microbiol2017; 67:889–895 [View Article]
XuP,
LiW-J,
TangS-K,
ZhangY-Q,
ChenG-Z et al.Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol2005; 55:1149–1153 [View Article]
BaurC,
KrewinkelM,
KranzB,
von NeubeckM,
WenningM et al. Quantification of the proteolytic and lipolytic activity of microorganisms isolated from raw milk. International Dairy Journal2015; 49:23–29 [View Article]
TindallBJ.
A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol1990; 13:128–130 [View Article]
TindallBJ,
SmibertRM,
KriegNR.
Phenotypic characterization and the principles of comparative systematics. In
ReddyC,
BeveridgeT,
BreznakJA,
MarzlufG,
SchmidtTM.
(editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007 pp 330–393