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Abstract

A Gram-stain-negative bacterium, designated strain PF-30, was isolated from floodwater of a paddy field in South Korea. Strain PF-30 was found to be a strictly aerobic, motile and pink-pigmented rods which can grow at 25–40 °C (optimum, 28 °C), at pH 5.0–9.0 (optimum pH 7.0) and at salinities of 0.5–3.0 % NaCl (optimum 0.5 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain PF-30 belongs to the genus , showing highest sequence similarity to TU-7 (97.1%) and less than 91.3 % similarity with other members of the family . The average nucleotide identity (ANI) and DNA–DNA relatedness between the strain PF-30 and TU-7 yielded an ANI value of 75.1 % and DNA–DNA relatedness of 11.7±0.7 %, respectively. The major fatty acids were identified as C and C 7. The predominant respiratory quinone was identified as Q-10. The DNA G+C content was determined to be 69.9 mol%. The strain PF-30 was observed to produce plant-growth-promoting materials such as indole-3-acetic acid (IAA), siderophore and phytase. On the basis of the results from phylogenetic, chemotaxonomic and phenotypic data, we concluded that strain PF-30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PF-30 (=KACC 19985=NBRC 113984).

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2020-02-06
2020-05-27
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