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Volume 70, Issue 3

sp. nov., isolated from an island soil Free

Abstract

A mycolic acid-containing actinobacterium designated strain MMS17-SY073 was isolated from island soil. The isolate showed best growth at 25 °C, pH 6, and 0 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MMS17-SY073 belongs to the genus , and is mostly related to the type strains of (98.5 % sequence similarity), (98.1%), and (97.8%). The genome-based comparisons showed a clear distinction between the strain and the two neighbouring species, and , with the average nucleotide identities (ANI) of 75.8 and 76.3 %, respectively. Notably, the genome of strain MMS17-SY073 was the largest in total stretch and gene counts among the complete genomes of , and contained a number of biosynthetic gene clusters for secondary metabolites, in particular those for non-ribosomal peptide synthetases. The major polar lipids were diphosphatidyl glycerol (DPG), phosphatidyl glycerol (PG), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI) and phosphatidyl inositol mannoside (PIM). The isoprenoid quinone was MK-9(H), and the main fatty acids were C (30.2%) and 10-methyl-C (33.7%). The whole cell hydrolysates contained galactose, arabinose, and -diaminopimelic acid. The DNA G+C content was 67.4 mol%. Based on phenotypic, chemotaxonomic and genetic analysis, strain MMS17-SY073 should be classified as a new species of the genus , for which the name sp. nov. is proposed (type strain=MMS17-SY073=KCTC 49257=JCM 33277).

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  • Accepted:
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  • Published Online:
Keyword(s): actinobacterium , biosynthetic gene cluster , Gordonia insulae and island soil
© 2020 The Authors
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2020-01-29
2024-04-23
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Gordonia insulae sp. nov., isolated from an island soil
Int J Syst Evol Microbiol 70, 2079 (2020); https://doi.org/10.1099/ijsem.0.004023
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