1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov. and sp. nov., two ascomycetous yeast species related to the clade isolated in Taiwan Free

Abstract

Four strains of anamorphic yeasts isolated from the fruiting bodies of mushrooms collected in Taiwan were found to represent two novel yeast species belonging to the genus , which was formally known as the clade. Strains NY13M09 and NY14M14 were related to the type strains of , , and , and strains GG4M07 and GG6M14 were related to NRRL Y-17087 and NRRL Y-27777. However, strains NY13M09 and NY14M14 differed from their closest phylogenetic neighbours by 2.9–3.7 % in the D1/D2 domain sequence of the LSU rRNA gene and by 6.6–13.7 % in the internal transcribed spacer (ITS); GG4M07 and GG6M14 differed from their closest known species by 2.4 % in the D1/D2 domain sequence of the LSU rRNA gene and by 8.7–10.0 % in the ITS. Meanwhile, these strains were also clearly distinguished from their closest relatives based on the results of physiological tests. Based on the characteristics described above, the strains could be regarded as representing two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The holotypes are BCRC 23475 and BCRC 23476.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Candida kruisii clade , fruiting bodies of mushrooms and Teunomyces
Funding
This study was supported by the:
  • Ministry of Science and Technology (Award MOST 107-2923-B-007-001-MY3)
    • Principle Award Recipient: Ching-Fu Lee
© 2020 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004021
2020-01-29
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/3/2103.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004021&mimeType=html&fmt=ahah

References

  1. Suh S-O, Nguyen NH, Blackwell M. A yeast clade near Candida kruisii uncovered: nine novel Candida species associated with basidioma-feeding beetles. Mycol Res 2006; 110:1379–1394 [View Article]
     [Google Scholar]
  2. Middelhoven WJ, Kurtzman CP. Four novel yeasts from decaying organic matter: Blastobotrys robertii sp. nov., Candida cretensis sp. nov., Candida scorzettiae sp. nov. and Candida vadensis sp. nov. Antonie van Leeuwenhoek 2007; 92:233–244 [View Article]
     [Google Scholar]
  3. Wang Y, Ren Y-C, Zhang Z-T, Wu F-H, Ke T et al. Candida funiuensi sp. nov., a cellobiose-fermenting yeast species isolated from rotten wood. Int J Syst Evol Microbiol 2015; 65:1755–1758 [View Article]
     [Google Scholar]
  4. Kurtzman CP, Robnett CJ, Blackwell M. Description of Teunomyces gen. nov. for the Candida kruisii clade, Suhomyces gen. nov. for the Candida tanzawaensis clade and Suhomyces kilbournensis sp. nov. FEMS Yeast Res 2016; 16:fow041–049 [View Article]
     [Google Scholar]
  5. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP et al. Candida Berkhout. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp 987–1278
     [Google Scholar]
  6. Hsieh C-W, Huang L-Y, Tschen EF-T, Chang C-F, Lee C-F. Five novel anamorphic, ascomycetous yeast species associated with mushrooms and soil. FEMS Yeast Res 2010; 10:948–956 [View Article]
     [Google Scholar]
  7. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp 137–144
     [Google Scholar]
  8. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article]
     [Google Scholar]
  9. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: a Guide to Methods and Applications New York: Academic Press; 1990 pp 315–322
     [Google Scholar]
  10. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article]
     [Google Scholar]
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article]
     [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  13. Barnett JA, Payne RW, Yarrow D. Yeasts, Characteristics and Identification, 3rd ed. Cambridge: Cambridge University Press; 2000
     [Google Scholar]
  14. Boutroux L. Sur La conservation des ferments alcooliques dans La nature. Ann Sci Nat IV Ser Bot Tome 1884; 17:145–209
     [Google Scholar]
  15. Suh SO, Blackwell M. The beetle gut as a habitat for new species of yeasts. In Vega FE, Blackwell M. (editors) Insect Fungal Associations: Ecology and Evolution New York: Oxford University Press; 2005 pp 244–256
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004021
Loading
Teunomyces basidiocarpi sp. nov. and Teunomyces luguensis sp. nov., two ascomycetous yeast species related to the Candida kruisii clade isolated in Taiwan
Int J Syst Evol Microbiol 70, 2103 (2020); https://doi.org/10.1099/ijsem.0.004021
/content/journal/ijsem/10.1099/ijsem.0.004021
/content/journal/ijsem/10.1099/ijsem.0.004021
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error