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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov., isolated from biological soil crusts Free

Abstract

A pink-pigmented, Gram-negative, rod-shaped, obligate aerobic bacterial strain, MIMD6, was isolated from biological soil crusts in PR China. Cells grew at 20–37 °C (optimum, 30 °C), at pH 6–8 (optimum, pH 7) and with 0–1 % (w/v) NaCl (optimum, 0 %). Strain MIMD6 could use methanol or formate as a sole carbon source to grow, and carried methanol dehydrogenase genes and , supporting its methylotrophic metabolism. The respiratory quinone was ubiquinone Q-10, the major fatty acids were Cω7 (87.3 %), and the major polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unknown aminolipid and one unidentified glycolipid. The results of phylogenetic analyses based on the sequences of the 16S rRNA gene, seven housekeeping genes (, , , , , and ) and methanol dehydrogenase genes indicated that strain MIMD6 formed a phylogenetic linage with members of the genus . Strain MIMD6 was most closely related to DSM 17168 and LMG 21967 with 16S rRNA gene sequence similarities of 95.7 and 95.2 %, respectively. The genomic DNA G+C content calculated via draft genome sequencing was 73.0 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain MIMD6 and the type strains of other species were 70.7–82.0 and 24.6–30.0 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain MIMD6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MIMD6 (=KCTC 52305=MCCC 1K01311).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Alphaproteobacteria , biological soil crusts , Methylobacteriaceae , Methylobacterium and Rhizobiales
Funding
This study was supported by the:
  • Special Fund for Nation-oriented Science and Technology Development of Region (Award 2017)
    • Principle Award Recipient: Fu ying Feng
  • Science and Technology Innovation-oriented Project of Inner Mongolia Autonomous Region (Award 2017)
    • Principle Award Recipient: Fu ying Feng
  • National Natural Science Foundation of China (Award 31560030)
    • Principle Award Recipient: Fu ying Feng
© 2020 The Authors
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2020-01-29
2024-12-07
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Methylobacterium crusticola sp. nov., isolated from biological soil crusts
Int J Syst Evol Microbiol 70, 2089 (2020); https://doi.org/10.1099/ijsem.0.004020
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