1887

International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 4

sp. nov., isolated from activated sludge Free

Abstract

A Gram-stain-negative, aerobic, non-flagellated and filamentous-shaped bacterium, HX-16-21, was isolated from activated sludge. Strain HX-16-21 was able to degrade gentisate, protocatechuic acid and -hydroxybenzoic acid and herbicides quizalofop-p-ethyl and diclofop-methyl. The strain shared 97.2 % 16S rRNA gene sequence similarity to CCTCC AB 2015052 and less than 97 % similarities to other type strains. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HX-16-21 belonged to the genus and formed a subclade with CCTCC AB 2015052. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and six unidentified lipids. The major fatty acids were iso-C, iso-C G and iso-C 3-OH. The predominant respiratory quinone was menaquinone 7 (MK-7). The draft genome of strain HX-16-21 was 8.1 Mb, and the G+C content was 43.5 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain HX-16-21 and CCTCC AB 2015052 were 80.6 and 26.8 %, respectively. Based on both phenotypic and phylogenetic evidence, strain HX-16-21 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is HX-16-21 (=KCTC 72288=ACCC 61580).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , Niastella caeni sp. nov. , polyphasic taxonomy and sludge
Funding
This study was supported by the:
  • Jian He , National Natural Science Foundation of China , (Award 31770117)
© 2020 The Authors
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2020-02-10
2021-03-08
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Niastella caeni sp. nov., isolated from activated sludge
Int J Syst Evol Microbiol 70, 2261 (2020); https://doi.org/10.1099/ijsem.0.004017
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