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Abstract

An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111, was isolated from faeces from a healthy Korean. Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111 was closely related to CCRI-16110 (93.9 %) and GAM6-1 (93.7 %), followed by DSM 1787 (93.5 %), ATCC 27340 (93.4 %), DSM 10507 (93.1 %) and ATCC 29236 (93.1 %) within the family ( rRNA cluster XIVa). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111 formed a separate branch with species in the genus . The major cellular fatty acids (>10.0 %) were C and C 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids. KGMB01111 contained diaminopimelic acid in cell-wall peptidoglycan. The predominant end product of fermentation produced by KGMB01111 was acetic acid. Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111 represents a novel species within the genus for which the name sp. nov. is proposed. The type strain is KGMB01111 (=KCTC 15706=DSM 107827).

Funding
This study was supported by the:
  • Seung-Hwan Park , National Research Foundation of Korea , (Award NRF-2014R1A6A3A04057492)
  • Jung-Sook Lee , National Research Foundation of Korea , (Award NRF-2016M3A9F3946674)
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2020-02-20
2020-06-02
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