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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

gen. nov., sp. nov., a member of order , isolated from human blood Free

Abstract

The taxonomic position of an unknown bacterial strain designated CNM695-12, isolated from the blood of an immunocompromised subject, was investigated via phenotypic, chemotaxonomic, genotypic and genomic analyses. Bacterial cells were determined to be Gram-stain-negative bacilli, aerobic, non-motile and non-spore-forming. The strain showed catalase activity but no oxidase activity. Optimal growth occurred at 37 °C, pH 7 and with 0–1 % NaCl. C, summed feature 8 (comprising Cω7C ω6), and Cω9 were the most abundant fatty acids, and ubiquinone 8 was the major respiratory quinone. The polar lipids present included phosphatidylglycerol, phosphatidylethanolamine and other aminophospholipids. The 16S rRNA gene sequence showed approximately 93.5 % similarity to those of different species with validly published names within the order (e.g. Feox-1, B8 B4 and K14). Phylogenetic analyses based on 16S rRNA gene sequences and concatenated alignments including the sequences for 107 essential proteins, revealed the strain to form a novel lineage close to members of the family . The highest average nucleotide identity and average amino acid identity values were obtained with K14 (69.6 and 55.7 % respectively). The genome, with a size of 3.35 Mb, had a DNA G+C content of 52.4 mol% and encoded 3056 predicted genes, 3 rRNA, 1 transfer–messengerRNA and 51 tRNA. Strain CNM695-12 thus represents a novel species belonging to a novel genus within the order , for which the name gen. nov., sp. nov. is proposed. The type strain is CNM695-12 (=DSM 104959=CECT 9208).

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  • Published Online:
Keyword(s): Betaproteobacteria , Burkholderiales , Comamonadaceae , Saezia and sanguinis
© 2020 The Authors
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2020-01-31
2024-04-18
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Saezia sanguinis gen. nov., sp. nov., a Betaproteobacteria member of order Burkholderiales, isolated from human blood
Int J Syst Evol Microbiol 70, 2016 (2020); https://doi.org/10.1099/ijsem.0.004010
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