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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov., a novel member of the genus isolated from marine sponge Free

Abstract

A taxonomic study was carried out on strain HN-E26, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E26 were Gram-stain-negative, motile by gliding, yellow-pigmented and rod-shaped. The strain could grow at 10–40 °C (optimum, 25 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in 0.5–12 % (w/v) NaCl (optimum, 4–7 %). This isolate was positive for oxidase, catalase, and the hydrolysis of starch, xylan, aesculin and gelatin, but negative for indole production and the reduction of nitrate. Strain HN-E26 shared the highest 16S rRNA gene sequence similarity with LYYY01 (95.5 %), followed by A2 (94.4 %), NH57N (94.3 %) and 022-2-26 (94.3 %). The phylogenetic tree based on 16S rRNA gene sequences revealed that strain HN-E26 formed a distinct phylogenetic lineage within the cluster comprising LYYY01 and ‘’ ST2L12. The dominant fatty acids were iso-C and iso-C G. The major polar lipids comprised phosphatidylethanolamine, three unidentified aminolipids and six unidentified lipids. The respiratory lipoquinone was identified as MK-6. The G+C content of the genomic DNA was 33.9 mol%. Based on the phenotypic and phylogenetic data, strain HN-E26 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain HN-E26 (=MCCC 1K03326=LMG 30458).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Mangrovimonas spongiae sp. nov , marine sponge and taxonomy
Funding
This study was supported by the:
  • the Young and the Middle-Aged Teacher Education Scientific Research Project of Fujian Province (Award No. JT180644)
    • Principle Award Recipient: Lingping Zhuang
  • the Scientific Research Project of Xiamen Southern Ocean Center (Award No.17GYY008NF04)
    • Principle Award Recipient: Lianzhong Luo
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.004006
2020-01-24
2023-05-29
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References

  1. Li Y, Bai S, Yang C, Lai Q, Zhang H et al. Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2013; 63:2043–2048 [View Article]
     [Google Scholar]
  2. Dinesh B, Furusawa G, Amirul AA. Mangrovimonas xylaniphaga sp. nov. isolated from estuarine mangrove sediment of Matang mangrove forest, Malaysia. Arch Microbiol 2017; 199:63–67 [View Article]
     [Google Scholar]
  3. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–IN1 [View Article]
     [Google Scholar]
  4. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
     [Google Scholar]
  5. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article]
     [Google Scholar]
  6. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
     [Google Scholar]
  7. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
     [Google Scholar]
  8. Bernardet J-F, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article]
     [Google Scholar]
  9. Hansen GH, Sørheim R. Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 1991; 13:231–241 [View Article]
     [Google Scholar]
  10. Smibert RM. Phenotypic Characterization. Methods for General and Molecular Bacteriology ; 1994
  11. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids; 1990
  12. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  13. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article]
     [Google Scholar]
  14. Groth I, Schumann P, Weiss N, Martin K, Rainey FA. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46:234–239 [View Article]
     [Google Scholar]
  15. Wang B, Sun F, Du Y, Liu X, Li G et al. Meridianimaribacter flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 2010; 60:121–127 [View Article]
     [Google Scholar]
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Mangrovimonas spongiae sp. nov., a novel member of the genus Mangrovimonas isolated from marine sponge
Int J Syst Evol Microbiol 70, 1982 (2020); https://doi.org/10.1099/ijsem.0.004006
/content/journal/ijsem/10.1099/ijsem.0.004006
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