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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 3

sp. nov., a novel member of the genus isolated from marine sponge Free

Abstract

A taxonomic study was carried out on strain HN-E26, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E26 were Gram-stain-negative, motile by gliding, yellow-pigmented and rod-shaped. The strain could grow at 10–40 °C (optimum, 25 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in 0.5–12 % (w/v) NaCl (optimum, 4–7 %). This isolate was positive for oxidase, catalase, and the hydrolysis of starch, xylan, aesculin and gelatin, but negative for indole production and the reduction of nitrate. Strain HN-E26 shared the highest 16S rRNA gene sequence similarity with LYYY01 (95.5 %), followed by A2 (94.4 %), NH57N (94.3 %) and 022-2-26 (94.3 %). The phylogenetic tree based on 16S rRNA gene sequences revealed that strain HN-E26 formed a distinct phylogenetic lineage within the cluster comprising LYYY01 and ‘’ ST2L12. The dominant fatty acids were iso-C and iso-C G. The major polar lipids comprised phosphatidylethanolamine, three unidentified aminolipids and six unidentified lipids. The respiratory lipoquinone was identified as MK-6. The G+C content of the genomic DNA was 33.9 mol%. Based on the phenotypic and phylogenetic data, strain HN-E26 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain HN-E26 (=MCCC 1K03326=LMG 30458).

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  • Accepted:
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  • Published Online:
Keyword(s): Mangrovimonas spongiae sp. nov , marine sponge and taxonomy
Funding
This study was supported by the:
  • the Young and the Middle-Aged Teacher Education Scientific Research Project of Fujian Province (Award No. JT180644)
    • Principle Award Recipient: Lingping Zhuang
  • the Scientific Research Project of Xiamen Southern Ocean Center (Award No.17GYY008NF04)
    • Principle Award Recipient: Lianzhong Luo
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.004006
2020-01-24
2025-04-29
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Mangrovimonas spongiae sp. nov., a novel member of the genus Mangrovimonas isolated from marine sponge
Int J Syst Evol Microbiol 70, 1982 (2020); https://doi.org/10.1099/ijsem.0.004006
/content/journal/ijsem/10.1099/ijsem.0.004006
/content/journal/ijsem/10.1099/ijsem.0.004006
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