In the course of screening halophilic bacteria in Urmia Lake in Iran, which is being threatened by dryness, a novel Gram-negative, moderately halophilic, heterotrophic and short rod-shaped bacteria was isolated and characterized. The bacterium was isolated from a water specimen and designated as TBZ3T. Colonies were found to be creamy yellow, with catalase- and oxidase-positive activities. The growth of strain TBZ3T was observed to be at 10–45 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 0.5–20 % (w/v) NaCl (optimum, 7.5 %). Strain TBZ3T contained C16 : 0, cyclo-C19 : 0ω8c, summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c) and summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as major fatty acids and ubiquinone-9 as the only respiratory isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid, unidentified phospholipid and unidentified polar lipids were detected as the major polar lipids. Strain TBZ3T was found to be most closely related to Halomonas saccharevitans AJ275T, Halomonas denitrificans M29T and Halomonas sediminicola CPS11T with the 16S rRNA gene sequence similarities of 98.93, 98.15 and 97.60 % respectively and in phylogenetic analysis strain TBZ3T grouped with Halomonas saccharevitans AJ275T contained within a large cluster within the genus Halomonas. Based on phenotypic, chemotaxonomic and molecular properties, strain TBZ3T represents a novel species of the Halomonas genus, for which the name Halomonas urmiana sp. nov. is proposed. The type strain is TBZ3T (=DSM 22871T=LMG 25416T).
VreelandRH,
LitchfieldCD,
MartinEL,
ElliotE,
elongataH.
A new genus and species of extremely salt-tolerant bacteria. Int J Syst Evol Microbiol1980; 30:485–495
GaboyerF,
Vandenabeele-TrambouzeO,
CaoJ,
CiobanuM-C,
JebbarM et al. Physiological features of Halomonas lionensis sp. nov., a novel bacterium isolated from a Mediterranean Sea sediment. Res Microbiol2014; 165:490–500 [View Article]
WangT,
WeiX,
XinY,
ZhuangJ,
ShanS et al.Halomonas lutescens sp. nov., a halophilic bacterium isolated from a lake sediment. Int J Syst Evol Microbiol2016; 66:4697–4704 [View Article]
VahedSZ,
ForouhandehH,
TarhrizV,
ChaparzadehN,
HejaziMA et al.Halomonas tabrizica sp. nov., a novel moderately halophilic bacterium isolated from Urmia Lake in Iran. Antonie van Leeuwenhoek2018; 111:1139–1148 [View Article]
LeeJ-C,
KimS-J,
WhangK-S.
Halomonas sediminicola sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment. Int J Syst Evol Microbiol2016; 66:3865–3872 [View Article]
VahedSZ,
ForouhandehH,
HassanzadehS,
KlenkH-P,
HejaziMA et al. Isolation and characterization of halophilic bacteria from Urmia lake in Iran. Microbiology2011; 80:834–841 [View Article]
Hossein MardiA,
KhaghaniA,
MacDonaldAB,
NguyenP,
KarimiN et al. The lake Urmia environmental disaster in Iran: a look at aerosol pollution. Sci Total Environ2018; 633:42–49 [View Article]
JeihouniM,
ToomanianA,
AlavipanahSK,
HamzehS.
Quantitative assessment of Urmia lake water using spaceborne multisensor data and 3D modeling. Environ Monit Assess2017; 189:572 [View Article]
SharifiA,
Shah-HosseiniM,
PourmandA,
EsfahaninejadM,
Haeri-ArdakaniO.
The vanishing of Urmia lake: a geolimnological perspective on the hydrological imbalance of the world’s second largest hypersaline lake. The Handbook of Environmental Chemistry2018; 1:38
RenY,
LingC,
HajnalI,
WuQ,
ChenG-Q.
Construction of Halomonas bluephagenesis capable of high cell density growth for efficient PHA production. Appl Microbiol Biotechnol2018; 102:4499–4510 [View Article]
TohmeS,
HacıosmanoğluGG,
EroğluMS,
KasaviC,
GençS et al.Halomonas smyrnensis as a cell factory for co-production of PHB and levan. Int J Biol Macromol2018; 118:1238–1246 [View Article]
LingC,
QiaoG-Q,
ShuaiB-W,
OlavarriaK,
YinJ et al. Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA). Metab Eng2018; 49:275–286 [View Article]
KirtelO,
MenéndezC,
VersluysM,
Van den EndeW,
HernándezL et al. Levansucrase from Halomonas smyrnensis AAD6T: first halophilic GH-J clan enzyme recombinantly expressed, purified, and characterized. Appl Microbiol Biotechnol2018; 102:9207–9220 [View Article]
MataJA,
BéjarV,
LlamasI,
AriasS,
BressollierP et al. Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis
. Res Microbiol2006; 157:827–835 [View Article]
Corti MonzónG,
NisenbaumM,
Herrera SeitzMK,
MurialdoSE.
New findings on aromatic compounds’ degradation and their metabolic pathways, the biosurfactant production and motility of the halophilic bacterium Halomonas sp. KHS3. Curr Microbiol2018; 75:1108–1118 [View Article]
HerisYS,
HajizadehN,
VahedSZ,
VallipourJ,
HejaziMA et al. Fe (III) reduction by Halomonas sp. TBZ9 and Maribobacter sp TBZ23, isolated from Urmia Lake in Iran. Adv Environ Biol2014; 8:59–66
VreelandRH,
MartinEL.
Growth characteristics, effects of temperature, and ion specificity of the halotolerant bacterium Halomonas elongata
. Can J Microbiol1980; 26:746–752 [View Article]
YoonS-H,
HaS-M,
KwonS,
LimJ,
KimY et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article]
WangQ,
GarrityGM,
TiedjeJM,
ColeJR.
Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol2007; 73:5261–5267 [View Article]
BankevichA,
NurkS,
AntipovD,
GurevichAA,
DvorkinM et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol2012; 19:455–477 [View Article]
LeeI,
Ouk KimY,
ParkS-C,
ChunJ.
OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol2016; 66:1100–1103 [View Article]
ChunJ,
OrenA,
VentosaA,
ChristensenH,
ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2018; 68:461–466 [View Article]
SmibertRA,
KriegNR.
Phenotypic characterization. In
GerhardtP.
editor Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
MinnikinDE,
O'DonnellAG,
GoodfellowM,
AldersonG,
AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241 [View Article]
MinnikinDE,
PatelPV,
AlshamaonyL,
GoodfellowM.
Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol1977; 27:104–117 [View Article]