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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

Description of sp. nov., isolated from a tepid spring Free

Abstract

China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001 and SY-13 were isolated. SYSU G01001 and SY-13 were Gram-stain-positive, motile and spore-forming. Colonies were white, circular, raised and punctiform. SYSU G01001 and SY-13 grew at pH 6.0–9.0 (optimum pH 8.0) and at 23–37 °C (optimum 28 °C). The 16S rRNA gene sequence similarity between SYSU G01001 and SY-13 was 99.6 % but these strains shared low sequence similarity with (97.5 %) indicating that they represented a novel species. On the basis of the results, SYSU G01001 was selected for further investigations and SY-13 was considered to represent a second strain of the species. The cell wall peptidoglycan of SYSU G01001 was -2,6-diaminopimelic acid and MK-7 was the only respiratory quinone. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L). The G+C content of the genomic DNA was 53.9 mol%. The average nucleotide identity (ANIb and ANIm) values between SYSU G01001 and LMG 29963 were below the cut-off level (95–96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. On the basis of the above results strain SYSU G01001 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU G01001 (=KCTC 33952=CGMCC 1.13870).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): genome analysis , hot springs and Paenibacillus tepidiphilus
Funding
This study was supported by the:
  • Key Realm R&D Program of Guangdong Province (Award 2018B020206001)
    • Principle Award Recipient: Wen-Jun Li
  • Natural Science Foundation of Guangdong Province, China (Award 2016A030312003)
    • Principle Award Recipient: Wen-Jun Li
© 2020 Sun Yat-Sen University, P.R. China
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2020-01-27
2024-04-19
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Description of Paenibacillus tepidiphilus sp. nov., isolated from a tepid spring
Int J Syst Evol Microbiol 70, 1977 (2020); https://doi.org/10.1099/ijsem.0.004004
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