@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004000, author = "Liu, Bin and Yang, Xiaojun and Sheng, Mengyao and Yang, Zhou and Qiu, Jiguo and Wang, Chenghong and He, Jian", title = "Sphingobacterium olei sp. nov., isolated from oil-contaminated soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "3", pages = "1931-1939", doi = "https://doi.org/10.1099/ijsem.0.004000", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004000", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Sphingobacterium olei sp. nov.", keywords = "16S rRNA gene", keywords = "Gram-stain-negative", keywords = "oil-contaminated soil", keywords = "taxonomy", abstract = "A Gram-stain-negative, rod-shaped, non-motile and non-spore-forming bacterium, designated HAL-9T, was isolated from oil-contaminated soil in Daqing oilfield, Heilongjiang Province, PR China. Strain HAL-9T was able to degrade quizalofop-p-ethyl and diclofop-methyl. Growth was observed at 10–35 °C (optimum, 30 °C), pH 6.0–10.0 (optimum, pH 7.0) and salinity of 0 %–5.0 % (w/v; optimum 1.0 %). The results of phylogenetic analysis based on the 16S rRNA gene indicated that strain HAL-9T belongs to the genus Sphingobacterium and showed the highest sequence similarity (98.3 %) to Sphingobacterium alkalisoli Y3L14T, followed by Sphingobacterium mizutaii DSM 11724T (95.1 %) and Sphingobacterium lactis DSM 22361T (95.1 %). Menaquinone-7 (MK-7) was the only isoprenoid quinone. The predominant cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C15: 0 and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three phosphoglycolipids and three unidentified lipids. The draft genome of strain HAL-9T was 5.41 Mb. The G+C content of strain HAL-9T was 40.6 mol%. Furthermore, the average nucleotide identity and in silico DNA–DNA hybridization values between strain HAL-9T and S. alkalisoli Y3L14T were 86.2 % and 32.8 %, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, genotypic and phylogenetic evidence, strain HAL-9T represents a novel species in the genus Sphingobacterium , for which the name Sphingobacterium olei sp. nov. is proposed. The type strain is HAL-9T (=ACCC 61581T=CCTCC AB 2019176T=KCTC 72287T).", }