1887

Abstract

A Gram-stain-negative, rod-shaped, non-motile and non-spore-forming bacterium, designated HAL-9, was isolated from oil-contaminated soil in Daqing oilfield, Heilongjiang Province, PR China. Strain HAL-9 was able to degrade quizalofop--ethyl and diclofop-methyl. Growth was observed at 10–35 °C (optimum, 30 °C), pH 6.0–10.0 (optimum, pH 7.0) and salinity of 0 %–5.0 % (w/v; optimum 1.0 %). The results of phylogenetic analysis based on the 16S rRNA gene indicated that strain HAL-9 belongs to the genus and showed the highest sequence similarity (98.3 %) to Y3L14, followed by DSM 11724 (95.1 %) and DSM 22361 (95.1 %). Menaquinone-7 (MK-7) was the only isoprenoid quinone. The predominant cellular fatty acids were summed feature 3 (C 7 and/or C 6), iso-C and iso-C 3-OH. The major polar lipids were phosphatidylethanolamine, three phosphoglycolipids and three unidentified lipids. The draft genome of strain HAL-9 was 5.41 Mb. The G+C content of strain HAL-9 was 40.6 mol%. Furthermore, the average nucleotide identity and DNA–DNA hybridization values between strain HAL-9 and Y3L14 were 86.2 % and 32.8 %, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, genotypic and phylogenetic evidence, strain HAL-9 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is HAL-9 (=ACCC 61581=CCTCC AB 2019176=KCTC 72287).

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2020-01-22
2020-02-28
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