%0 Journal Article %A Wu, Yen-Chi %A Lin, Shih-Ting %A Guu, Jia-Rong %A Tamura, Tomohiko %A Mori, Koji %A Wang, Li-Ting %A Huang, Lina %A Watanabe, Koichi %T Vagococcus silagei sp. nov., isolated from brewer’s grain used to make silage in Taiwan %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 3 %P 1953-1960 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003999 %K silage %K Vagococcus silagei %K brewer's grain %I Microbiology Society, %X A Gram-stain-positive, coccus- or oval-shaped, non-motile, haemolytic, asporogenous, catalase- and oxidase-negative, and facultatively anaerobic strain, 2B-2T, was isolated from a brewer’s grain used to make silage in Taiwan. Comparative analyses of 16S rRNA, hsp60 and pheS gene sequences demonstrated that strain 2B-2T was a member of the genus Vagococcus . On the basis of 16S rRNA gene sequence similarity, the type strains of Vagococcus teuberi (98.4 % similarity), Vagococcus carniphilus (98.4 %), Vagococcus martis (98.2 %), Vagococcus penaei (98.2 %) and Vagococcus fluvialis (98.0 %) were the closest neighbours to this novel strain. The similarity levels of concatenated housekeeping gene sequences (hsp60 and pheS) between strain 2B-2T and these closely related species ranged from 84.5 to 88.0 %. The average nucleotide identity and in silico DNA–DNA hybridization values between strain 2B-2T and its closest relatives were lower than 72.9 and 21.6 %, respectively. The DNA G+C content was 34.7 mol%. Phenotypic and genotypic features demonstrated that strain 2B-2T represents a novel species of the genus Vagococcus , for which the name Vagococcus silagei sp. nov. is proposed. The type strain is 2B-2T (=BCRC 81132T=NBRC 113536T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003999