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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov., isolated from brewer’s grain used to make silage in Taiwan Free

Abstract

A Gram-stain-positive, coccus- or oval-shaped, non-motile, haemolytic, asporogenous, catalase- and oxidase-negative, and facultatively anaerobic strain, 2B-2, was isolated from a brewer’s grain used to make silage in Taiwan. Comparative analyses of 16S rRNA, and gene sequences demonstrated that strain 2B-2 was a member of the genus . On the basis of 16S rRNA gene sequence similarity, the type strains of (98.4 % similarity), (98.4 %), (98.2 %), (98.2 %) and (98.0 %) were the closest neighbours to this novel strain. The similarity levels of concatenated housekeeping gene sequences ( and ) between strain 2B-2 and these closely related species ranged from 84.5 to 88.0 %. The average nucleotide identity and DNA–DNA hybridization values between strain 2B-2 and its closest relatives were lower than 72.9 and 21.6 %, respectively. The DNA G+C content was 34.7 mol%. Phenotypic and genotypic features demonstrated that strain 2B-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2B-2 (=BCRC 81132=NBRC 113536).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): brewer's grain , silage and Vagococcus silagei
Funding
This study was supported by the:
  • Ministry of Economic Affairs (TW) (Award 107-EC-17-A-22-0525)
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2020-01-31
2024-04-24
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Vagococcus silagei sp. nov., isolated from brewer’s grain used to make silage in Taiwan
Int J Syst Evol Microbiol 70, 1953 (2020); https://doi.org/10.1099/ijsem.0.003999
/content/journal/ijsem/10.1099/ijsem.0.003999
/content/journal/ijsem/10.1099/ijsem.0.003999
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