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Abstract

We performed taxonomic studies on a psychrotolerant strain, designated PB01, isolated from an Antarctic iceberg. The cells of strain PB01 were Gram-stain-positive, strictly aerobic, white–yellow and rod-shaped. The results of 16S rRNA gene sequence analysis revealed that strain PB01 was closely related to DSM 11713 (99.19 % similarity), DSM 11706 (98.91 %) and DSM 5 (98.85 %). Despite high 16S rRNA gene sequence similarity, the degrees of DNA–DNA relatedness between strain PB01 and its three closest phylogenetic neighbours were 62.4±7.3 % for DSM 11713, 61.1±5.4 % for DSM 11706 and 56.1±6.9 % for DSM 5. The predominant cellular fatty acids were anteiso-C, iso-C and C16 : 1ω7с-OH. Menaquinone-8 was the major respiratory quinone, and phosphatidylethanolamine was the major polar lipid. The DNA G+C content of strain PB01 calculated from the complete genome sequence was 36.0 mol%. Based on the phenotypic, chemotaxonomic, genomic and phylogenetic data obtained in the present study, we conclude that strain PB01 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is PB01 (=CECT 9792=KCTC 43041).

Keyword(s): Psychrobacillus , Antarctica and iceberg
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/content/journal/ijsem/10.1099/ijsem.0.003998
2020-01-27
2020-02-28
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