1887

Abstract

We performed taxonomic studies on a psychrotolerant strain, designated PB01, isolated from an Antarctic iceberg. The cells of strain PB01 were Gram-stain-positive, strictly aerobic, white–yellow and rod-shaped. The results of 16S rRNA gene sequence analysis revealed that strain PB01 was closely related to DSM 11713 (99.19 % similarity), DSM 11706 (98.91 %) and DSM 5 (98.85 %). Despite high 16S rRNA gene sequence similarity, the degrees of DNA–DNA relatedness between strain PB01 and its three closest phylogenetic neighbours were 62.4±7.3 % for DSM 11713, 61.1±5.4 % for DSM 11706 and 56.1±6.9 % for DSM 5. The predominant cellular fatty acids were anteiso-C, iso-C and C16 : 1ω7с-OH. Menaquinone-8 was the major respiratory quinone, and phosphatidylethanolamine was the major polar lipid. The DNA G+C content of strain PB01 calculated from the complete genome sequence was 36.0 mol%. Based on the phenotypic, chemotaxonomic, genomic and phylogenetic data obtained in the present study, we conclude that strain PB01 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is PB01 (=CECT 9792=KCTC 43041).

Keyword(s): Antarctica , iceberg and Psychrobacillus
Funding
This study was supported by the:
  • Ministry of Education (Award 2019R1A6A11051471)
    • Principle Award Recipient: Pyung Cheon Lee
  • Ministry of Education (Award 2017R1A2B4011899)
    • Principle Award Recipient: Pyung Cheon Lee
  • Ministry of Education (Award 2014M3A6A8066439)
    • Principle Award Recipient: Pyung Cheon Lee
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2020-01-27
2024-03-29
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References

  1. Larkin JM, Stokes JL. Isolation of psychrophilic species of Bacillus . J. Bacteriol 1966; 91:1667–1671
    [Google Scholar]
  2. Larkin JM, Stokes JL. Taxonomy of psychrophilic strains of Bacillus . J. Bacteriol 1967; 94:889–895
    [Google Scholar]
  3. Krishnamurthi S, Ruckmani A, Pukall R, Chakrabarti T. Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman, et al., 2002 and B. psychrodurans Abd-El Rahman, et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov. Syst Appl Microbiol 2010; 33:367–373
    [Google Scholar]
  4. Pham VH, Jeong SW, Kim J. Psychrobacillus soli sp. nov., capable of degrading oil, isolated from oil-contaminated soil. Int J Syst Evol Micrbiol 2015; 65:3046–3052
    [Google Scholar]
  5. Shen Y, Fu Y, Yu Y, Zhao J, Li J et al. Psychrobacillus lasiicapitis sp. nov., isolated from the head of an ant (Lasius fuliginosus). Int J Syst Evol Micrbiol 2017; 67:4462–4467
    [Google Scholar]
  6. Choi JY, Kim SC, Lee PC. Comparative genome analysis of Psychrobacillus strain PB01, isolated from an iceberg. J Microbiol Biotechnol 2020; accepted:
    [Google Scholar]
  7. Felske A, Akkermans AD, De Vos WM. Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis fingerprints. Appl Environ Microbiol 1998; 64:4581–4587
    [Google Scholar]
  8. Yoon SH, SM H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617
    [Google Scholar]
  9. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680
    [Google Scholar]
  10. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874
    [Google Scholar]
  11. Jukes TH, Cantor CR. Evolution of Protein Molecules. Mammalian Protein Metabolism 3 New York: Academic Press; 1969 pp 21–132
    [Google Scholar]
  12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791
    [Google Scholar]
  14. Ezaki T. Hashimoto, Yasuhiro, Yabuuchi, et al. fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 1989; 39:224–229
    [Google Scholar]
  15. McInnes JL, Forster AC, Skingle DC, Symons RH. Preparation and uses of photobiotin. Methods Enzymol 1990; 184:588–600
    [Google Scholar]
  16. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST server: rapid annotations using subsystems technology. BMC genomics 2008; 9:75
    [Google Scholar]
  17. Rodrigues DF, Tiedje JM. Coping with our cold planet. Appl Environ Microbiol 2008; 74:1677–1686
    [Google Scholar]
  18. De Maayer P, Anderson D, Cary C, Cowan DA. Some like it cold: understanding the survival strategies of psychrophiles. EMBO Rep 2014; 15:508–5017
    [Google Scholar]
  19. Kim M, HS O, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int. J. Syst. Evol. Microbiol 2014; 64:346–351
    [Google Scholar]
  20. Choi JY, Kim SC, Lee PC. Flavobacterium kingsejongi sp. nov., a carotenoid-producing species isolated from Antarctic penguin faeces. Int J Syst Evol Micrbiol 2018; 68:911–916
    [Google Scholar]
  21. WEBLEY DM. A simple method for producing microcultures in hanging drops with special reference to organisms utilizing oils. J. gen. Microbiol 1953; 8:66–71
    [Google Scholar]
  22. Reynolds J, Moyes R, Breakwell DP. Differential staining of bacteria: endospore stain. Curr. Protoc 2009; 3:3J
    [Google Scholar]
  23. McCammon SA, Bowman JP. Taxonomy of antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov . Int J Syst Evol Microbiol 2000; 50:1055–1063
    [Google Scholar]
  24. Lewin RA, Isolation LDM. cultivation and characterization of flexibacteria . J Gen Microbiol 1969; 58:145–170
    [Google Scholar]
  25. Nguyen TM, Kim J. A rapid and simple method for identifying bacterial polar lipid components in wet biomass. Journal of Microbiology 2017; 55:635–639
    [Google Scholar]
  26. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917
    [Google Scholar]
  27. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Evol Microbiol 1977; 27:104–117
    [Google Scholar]
  28. Yokota A, Tamura T, Hasegawa HLH. Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 1993; 43:805–812
    [Google Scholar]
  29. Kong MK, Lee PC. Metabolic engineering of menaquinone-8 pathway of Escherichia coli as a microbial platform for vitamin K production. Biotechnol Bioeng 2011; 108:1997–2002
    [Google Scholar]
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