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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov., an endophytic bacterial species isolated from the root of Smith var. Free

Abstract

A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325, was isolated from the root of Smith var. collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization. It grew optimally with 0–1 % NaCl (w/v), at pH 7 and at 30 °C. The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The major cellular fatty acid was anteiso-C. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to R-3 (99.0 %), CCTCC AB 2016047 (97.9 %), DSM 19417 (97.5 %) and LMG 27297 (97.2 %). The digital DNA–DNA hybridization and average nucleotide identity values between py1325 and these species ranged 20.6–53.3 % and 79.9–93.6 %. The G+C content of the genomic DNA was 47.7 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is py1325 (=CCTCC AB 2015220=LMG 29068).

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  • Accepted:
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Keyword(s): endophytes , Paenibacillus paridis sp. nov. and Paris polyphylla Smith var.yunnanensis
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 31560309)
    • Principle Award Recipient: Xiao-Yang Zhi
  • the National Natural Science Foundation of China (Award 31500007)
    • Principle Award Recipient: Ling-Ling Yang
  • Program for Excellent Young Talents, Yunnan University
    • Principle Award Recipient: Xiao-Yang Zhi
© 2020 The Authors
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2020-01-22
2024-04-20
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Paenibacillus paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis
Int J Syst Evol Microbiol 70, 1940 (2020); https://doi.org/10.1099/ijsem.0.003997
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