1887

Abstract

The taxonomic position of a novel actinomycete isolate, designated strain GGCR-6, isolated from the healthy leaves of collected from the botanic garden of Hunan University of Science and Technology in Hunan province, PR China, was determined by a polyphasic approach. GGCR-6 grew well on ISP series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into straight spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was -diaminopimelic acid. The major menaquinones were MK-9(H), MK-9(H), MK-9 and MK-9(H). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphotidylinositol and phosphatidylinositol mannosides. The predominant fatty acids were Cω9, iso-C and C. The phenotypic characteristics of GGCR-6 indicated that it represented a member of the genus . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that GGCR-6 was most closely related to NRRL B2296 and NRRL B1818. However, the digital DNA–DNA hybridization, the average nucleotide identity and the multi locus sequence analysis evolutionary distance clearly separate GGCR-6 from the phylogenetically closely related species. Furthermore, the novel isolate was distinctly differentiated from NRRL B2296 and NRRL B1818 by morphological, physiological and biochemical characteristics. Based on these data, strain GGCR-6 should be designated as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain GGCR-6 (=CICC 11055=JCM 33584).

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2020-01-22
2020-02-28
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