1887

Abstract

Strains Sr36 and TMT4-23 were isolated from No. 1 glacier in Xinjiang Uygur Autonomous Region and Toumingmengke glacier in Gansu Province, PR China, respectively. They were Gram-stain-positive and rod-shaped micro-organisms. The optimum growth temperature of the two strains was 10–14 °C. Phylogenetic analysis showed that the two strains were related to members of the genus . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain Sr36 and its close relatives Hh15, Hh31, Hh34 and Hh8 were 81.16–87.24 and 28.0–32.5 %, respectively. The ANI and dDDH values between strain TMT4-23 and its close relative 0549 were 81.16 and 22.3 %. The polar lipids of strain Sr36 were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The polar lipids of strain TMT4-23 were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid and six unidentified lipids. The major fatty acids of strain Sr36 were anteiso-C, iso-C, anteiso-C and anteiso-C. The major fatty acids of strain TMT4-23 were anteiso-C, anteiso-C, iso-C, anteiso-C and iso-C. Both strains contained 2,4-diaminobutyric acid and their predominant menaquinone was MK-10. On the basis of the phenotypic, phylogenetic and genotypic data, two novel species sp. nov. (type strain = Sr36=CGMCC 1.9275=NBRC 113797) and sp. nov. (type strain =TMT4-23=CGMCC 1.9556=NBRC 113800) are proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31670003)
    • Principle Award Recipient: Yu-Hua Xin
  • National Natural Science Foundation of China (Award 31600007)
    • Principle Award Recipient: Qing Liu
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2020-02-25
2024-12-05
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