1887

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Volume 70, Issue 4

sp. nov., isolated from plateau pika () Free

Abstract

Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-A and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai–Tibet Plateau. Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C. The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to , respectively. In the 16S rRNA gene and polygenetic trees, strains MF30-A and MF845 were clearly distinct from other species. The two strains could not produce acid from arbutin, -fructose, D-sucrose, glycogen, salicin or starch. Production of β-glucosidase by these strains was negative. The major fatty acids of these strains were anteiso-C, anteiso-C and iso-C. Strain MF30-A contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones. The major polar lipids in strain MF30-A were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid. The G+C contents of the DNA of strains MF30-A and MF845 were 69.8 mol% and 69.7 mol%, respectively. The average nucleotide identity and digital DNA–DNA relatedness values of the two strains with all available genomes of the genus were far below the respective thresholds of 95 and 70 %, respectively. All genotypic and phenotypic data indicated that strains MF30-A and MF845 should be classified as novel members of the genus , for which the name sp. nov. is proposed. The type strain is MF30-A (=CGMCC 1.16469=DSM 106183).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Agromyces badenianii , novel species and plateau pika
Funding
This study was supported by the:
  • Chinese Academy of Medical Sciences and Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: Jianguo Xu
  • National Science and Technology Major Project of China (Award 2018ZX10305409-003)
    • Principle Award Recipient: Zhihong Ren
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
© 2020 The Authors
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2020-02-25
2024-12-02
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Agromyces badenianii sp. nov., isolated from plateau pika (Ochotona curzoniae)
Int J Syst Evol Microbiol 70, 2247 (2020); https://doi.org/10.1099/ijsem.0.003987
/content/journal/ijsem/10.1099/ijsem.0.003987
/content/journal/ijsem/10.1099/ijsem.0.003987
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