RT Journal Article SR Electronic(1) A1 Kajale, Swapnil A1 Deshpande, Neelima A1 Pali, Snigdha A1 Shouche, Yogesh A1 Sharma, AvinashYR 2020 T1 Natrialba swarupiae sp. nov., a halophilic archaeon isolated from a hypersaline lake in India JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 3 SP 1876 OP 1881 DO https://doi.org/10.1099/ijsem.0.003986 PB Microbiology Society, SN 1466-5034, AB A haloarchaeal strain (ESP3B_9T) was isolated from the salt pan of Sambhar salt lake, Rajasthan, India. Cells were coccoid, non-motile, Gram-stain-negative and formed reddish-pink pigmented colonies. The strain was aerobic, able to grow at 35–55 °C (optimum, 40 °C), in 20–35 % (25 %) NaCl and pH 8–10 (pH 9). Mg2+ not required for growth. The cells were lysed in distilled water and the minimum NaCl concentration that prevented cell lysis was 5 % w/v. The 16S rRNA gene sequence similarities between strain ESP3B_9T and Natrialba hulunbeirensis JCM 10989T and Natrialba magadii ATCC 43099T were 96.53 and 96.25 % respectively. The similarities of the RNA polymerase subunit B gene between strain ESP3B_9T and N. hulunbeirensis JCM 10989T and N. magadii ATCC 43099T were 84.47 and 84.9 % respectively. Genome sequencing revealed a genome size of 4.20 Mbp with DNA G+C content of 62.5 mol%. The major polar lipids were phosphotidylglycerol and phosphatidylglycerol phosphate methyl esters with minor amounts of unidentified lipids. The results of polyphasic analysis determined that strain ESP3B_9T represents a novel species of the genus Natrialba , for which the name Natrialba swarupiae sp. nov. is proposed. The type strain is ESP3B_9T (MCC 3419T=JCM 33002T=KCTC 4279T=CGMCC 1.16737T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003986