A novel strain, designated AQ6-296T, was isolated from a soil sample collected in Fildes Peninsula, Antarctic. Cells were Gram-stain-negative, non-endospore-forming, non-motile, strictly aerobic and rod-shaped. Growth occurred at 4–28 °C (optimum, 20 °C) and at pH 6.0–7.0 (optimum, pH 7.0). NaCl was not obligatory for growth. Colonies were pale yellow after growth for 3 days at 20 °C on Reasoner's 2A agar. The strain was weakly positive for oxidase and the catalase test was negative. The only respiratory quinone was Q-8. The predominant cellular fatty acids were iso-C16 : 0, iso-C15 : 0, iso-C11 : 0 3OH, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (comprising iso-C17 : 1ω9c and/or C16 : 010-methyl). The major polar lipids were phosphatidylethanolamine, unknown aminolipids, phosphatidylglycerol and diphosphatidylglycerol. The results of phylogenetic analysis based on 16S rRNA gene sequences (the highest similarity at 92.4 % to Lysobacter dokdonensis) indicated that strain AQ6-296T is within the family Xanthomonadaceae. The DNA G+C content of the type strain was 58.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AQ6-296T is considered to represent a novel genus and species in the family Xanthomonadaceae, for which the name Pseudolysobacter antarcticus gen. nov., sp. nov. is proposed. The type strain is AQ6-296T (CCTCC AB 2016313T=KCTC 52744T).
SaddlerGS,
BradburyJF.
Family I. Xanthomonadaceae fam. nov. In
BrennerDJ,
KriegNR,
StaleyJT,
GarrityGM.
(editors) Bergey’s Manual of Systematic Bacteriology63, 2nd ed. New York: Springer; 2005
BusseHJ,
KämpferP,
MooreERB,
NuutinenJ,
TsitkoIV et al.Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. Int J Syst Evol Microbiol2002; 52:473–483 [View Article]
RaineyFA,
Ward-RaineyN,
KroppenstedtRM,
StackebrandtE.
The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol1996; 46:1088–1092 [View Article]
YoonS-H,
HaS-M,
KwonS,
LimJ,
KimY et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article]
KimuraM.
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol1980; 16:111–120 [View Article]
BowmanJP.
Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol2000; 50 Pt 5:1861–1868
DoetschRN.
Determinative methods of light microscopy. In
GerhardtP,
MurrayRGE,
CostilowRN,
NesterEW,
WoodWA.
(editors) Manual of Methods for General Bacteriology Washington, DC, USA: American Society for Microbiology; 1981 pp 21–33
BauerAW,
KirbyWM,
SherrisJC,
TurckM.
Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol1966; 45:493–496 [View Article]
SmibertRM,
KriegNR.
Phenotypic characterization. In
GerhardtP,
MurrayRGE,
WoodWA,
KriegNR.
(editors) Methods for General and Molecular Bacteriology Washington, DC: American society for Microbiology; 1994 pp 607–654
MooreDD,
DowhanD.
Preparation and analysis of DNA. In
AusubelFW,
BrentR,
KingstonRE,
MooreDD,
SeidmanJG.
(editors) Current Protocols in Molecular Biology New York: Wiley; 1995 pp 2–11
MesbahM,
PremachandranU,
WhitmanWB.
Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol1989; 39:159–167 [View Article]
WangL,
ZhengS,
WangD,
WangL,
WangG.
Thermomonas carbonis sp. nov., isolated from the soil of a coal mine. Int J Syst Evol Microbiol2014; 64:3631–3635 [View Article]
ParkJH,
KimR,
AslamZ,
JeonCO,
ChungYR.
Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter
. Int J Syst Evol Microbiol2008; 58:387–392 [View Article]
YuT-T,
ZhouE-M,
YinY-R,
YaoJ-C,
MingH et al.Vulcaniibacterium tengchongense gen. nov., sp. nov. isolated from a geothermally heated soil sample, and reclassification of Lysobacter thermophilus Wei et al. 2012 as Vulcaniibacterium thermophilum comb. nov. Antonie van Leeuwenhoek2013; 104:369–376 [View Article]
LeeEM,
JeonCO,
ChoiI,
ChangK-S,
KimC-J.
Silanimonas lenta gen. nov., sp. nov., a slightly thermophilic and alkaliphilic gammaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol2005; 55:385–389 [View Article]