1887

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Volume 70, Issue 3

sp. nov., a alkaliphilic bacterium from the rhizosphere of Free

Abstract

A novel bacterial strain, designated AK13 (=KACC 21401=DSM 109981), was isolated from the rhizosphere of . Strain AK13 was found to be an aerobic, Gram-stain–positive, endospore-forming and rod-shaped bacterium. It formed yellow circular colonies with smooth convex surfaces. The genomic DNA G+C content of strain AK13 was estimated to be 40 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to MLB2 (99.4 %), K11 (98.8 %) and PAT 05 (96.6 %). The average nucleotide identity values between strain AK13 and MLB2, K11 and PAT 05 were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA–DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively. Cells grew at 5–40 °C (optimum, 28–35 °C), pH 6.5–13 (optimum, pH 8–9) and in the presence of 0–13.0 % (w/v) NaCl (optimum, 1 %). The cell wall of strain AK13 contained -diaminopimelic acid, and the major isoprenoid quinone was MK-7. Results of fatty acid methyl ester analysis revealed that iso-C was the predominant cellular fatty acid. Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid. The genotypic and phenotypic characteristics suggested that strain AK13 represented a novel species of the genus , and thus the name sp. nov. is proposed.

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  • Accepted:
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  • Published Online:
Keyword(s): alkaliphilic , Bacillus , calcium carbonate , endospore , halotolerant and taxonomy
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR201902111)
    • Principle Award Recipient: Byoung-Hee Lee
  • Ministry of Land, Infrastructure and Transport (Award 19SCIP-B103706-05)
    • Principle Award Recipient: Woojun Park
© 2020 The Authors
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2020-01-17
2024-04-20
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Bacillus miscanthi sp. nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus
Int J Syst Evol Microbiol 70, 1843 (2020); https://doi.org/10.1099/ijsem.0.003982
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