1887

Abstract

Two novel, strictly aerobic, sulfur-dependent, thermoacidophilic strains, IC-006 and IC-007, were isolated from a solfataric field at Hakone Ohwaku-dani, Kanagawa, Japan. Cells of the two strains were irregular cocci with a diameter of 1.0–1.8 µm. They were strict aerobes and grew in a temperature range between 45 and 69 °C (optimally at 65 °C) and a pH range between 0.4 and 5.5 (optimally at pH 3.5). They required sulfur or a reduced sulfur compound, and sulfur was oxidized to sulfate. They grew autotrophically or mixotrophically utilizing several sugars and complex organic substances as carbon sources. The DNA G+C content was 42.4 mol%. A comparison of the 16S rRNA gene sequences among members of the order indicated that they were closely related to , forming an independent lineage within this order. The two isolates and were also diffentiated based on their phenotypic properties from the other members of the order . Detailed comparisons of the phenotypic properties and DNA–DNA hybridization study illustrated that the two isolates belong to a species different from . On the basis of the phylogenetic and phenotypic comparisons, we propose a new genus and species, gen. nov., sp. nov. to accommodate strains IC-006 and IC-007. The type strain of is IC-006 (=JCM 16833=DSM 104736). In addition, should be transferred to the new genus as comb. nov.: the type strain is Kra23 (=DSM 6482=JCM 9184=NBRC 15436).

Funding
This study was supported by the:
  • Shingo Kato , Japan Society for the Promotion of Science , (Award JP19H03310)
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2020-01-20
2020-04-08
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