1887

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Volume 70, Issue 3

sp. nov., belonging to the class , isolated from soil of the Mt Zao volcano Free

Abstract

An aerobic, Gram-stain-positive, mesophilic strain, W12, was isolated from soil of the Mt Zao volcano in Miyagi, Japan. Cells were filamentous, non-motile, and grew at 20–37 °C (optimally at 30 °C), at pH 5.0–7.0 (optimally at pH 6.0) and with <2 % (w/v) NaCl on 10-fold diluted Reasoner’s 2A (R2A) medium. Oval-shaped spores were formed on aerial mycelia. Strain W12 hydrolysed microcrystalline cellulose and xylan very weakly, and used -glucose as its sole carbon source. The major menaquinone was MK-9, and the major cellular fatty acids were C 2-OH, iso-C, summed feature 9 (10-methyl C and/or iso-Cω9) and anteiso-C. Cell-wall sugars were mannose and xylose, and cell-wall amino acids were -glutamic acid, glycine, -serine, -alanine, -alanine, β-alanine and -ornithine. Polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. Strain W12 has a genome of 7.42 Mb with 49.7 mol% G+C content. Nine copies of 16S rRNA genes with a maximum dissimilarity of 1.02 % and 13 biosynthetic gene clusters mainly coding for peptide products were predicted in the genome. Phylogenetic analysis based on both 16S rRNA gene and whole genome sequences indicated that strain W12 represents a novel species in the genus . The most closely related type strain was Uno16, with 16S rRNA gene sequence similarity and genomic average nucleotide identity of 98.37 % and 80.00 %, respectively. Herein, we propose the name sp. nov. for the type strain W12 (=NBRC 113551=BCRC 81169) in the bacterial class .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): class Ktedonobacteria , Dictyobacter vulcani sp. nov , polyphasic taxonomy and whole genome sequencing
Funding
This study was supported by the:
  • China Scholarship Council (Award 201606330094)
    • Principle Award Recipient: Yu Zheng
  • MEXT/JSPS KAKENHI (Award 18K05406 and 16H06279)
    • Principle Award Recipient: Shuhei Yabe
© 2020 The Authors
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2020-01-23
2024-04-24
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Dictyobacter vulcani sp. nov., belonging to the class Ktedonobacteria, isolated from soil of the Mt Zao volcano
Int J Syst Evol Microbiol 70, 1805 (2020); https://doi.org/10.1099/ijsem.0.003975
/content/journal/ijsem/10.1099/ijsem.0.003975
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