1887

Abstract

A novel strain (SSL-25) was isolated from mangrove soil sampled at QinzhouBay, PR China. The isolate was observed to be Gram-stain-positive and to form greyish-white aerial mycelia that differentiated into straight spore chains with smooth-surfaced spores on International Project 2 medium. The cell-wall peptidoglycan was determined to contain ll-diaminopimelicacid. The cell-wall sugars were glucose and mannose. The predominant menaquinones were MK-9 (H6), MK-9 (H8) and MK-9 (H4). The major polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and several unidentified phospholipids. The predominant cellular fatty acids were C, iso-C and summed feature 3 (Cω7/Cω6). The genome size of strain SSL-25 was 8.1 Mbp with a G+C content of 71.5 mol%. Phylogenetic analysis indicated that strain SSL-25 is closely related to NRRL 18488 (99.4 % sequence similarity). However, the digital DNA–DNA hybridization (39.8 %) and average nucleotide identity (91.3 %) values between them showed that it represents a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain SSL-25 from NRRL 18488. Therefore, based on these results, it is concluded that strain SSL-25 represents a novel species, for which the name sp. nov. is proposed. The type strain is SSL-25 (=CICC 11054=JCM33585).

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2020-01-17
2020-02-28
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References

  1. Harvey A. Natural products in drug discovery. Drug Discov Today 2008;13: 894– 901 [CrossRef]
    [Google Scholar]
  2. Zhang L, An R, Wang J, Sun N, Zhang S et al. Exploring novel bioactive compounds from marine microbes. Curr Opin Microbiol 2005;8: 276– 281 [CrossRef]
    [Google Scholar]
  3. Bérdy J. Thoughts and facts about antibiotics; where we are now and where are we heading. J Antibiot 2012;51: 1– 26
    [Google Scholar]
  4. Ashton EC, Macintosh DJ, Hogarth PJ. A baseline study of the diversity and community ecology of crab and molluscan macrofauna in the Sematan mangrove forest, Sarawak, Malaysia. J Trop Ecol 2003;19: 127– 142 [CrossRef]
    [Google Scholar]
  5. Azman A-S, Othman I, Velu SS, Chan K-G, Lee L-H. Mangrove rare actinobacteria: taxonomy, natural compound, and discovery of bioactivity. Front Microbiol 2015;6: 856 [CrossRef]
    [Google Scholar]
  6. Lee L-H, Zainal N, Azman A-S, Eng S-K, Ab Mutalib N-S et al. Streptomyces pluripotens sp. nov., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Staphylococcus aureus. Int J Syst Evol Microbiol 2014;64: 3297– 3306 [CrossRef]
    [Google Scholar]
  7. Ser H-L, Palanisamy UD, Yin W-F, Chan K-G, Goh B-H et al. Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines. Sci Rep 2016;6: 24247 [CrossRef]
    [Google Scholar]
  8. Ser H-L, Tan LT-H, Palanisamy UD, Abd Malek SN, Yin W-F et al. Streptomyces antioxidans sp. nov., a novel mangrove soil actinobacterium with antioxidative and neuroprotective potentials. Front Microbiol 2016;7: 899 [CrossRef]
    [Google Scholar]
  9. JWF L, Ser HL, Duangjai A, Saokaew S, Bukhari SI et al. Streptomyces colonosanans sp. nov., a novel actinobacterium isolated from Malaysia mangrove soil exhibiting antioxidative activity and cytotoxic potential against human colon cancer cell lines. Front Microbiol 2017;8: 877
    [Google Scholar]
  10. JWF L, Ser HL, Ab Mutalib NS, Saokaew S, Duangjai A et al. Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential. Sci Rep 2019;9: 3056
    [Google Scholar]
  11. DN H, Gao C, Sun CH, Jin T, Fan GY et al. Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China. Sci Rep 2019;9: 823
    [Google Scholar]
  12. Ser H-L, Zainal N, Palanisamy UD, Goh B-H, Yin W-F et al. Streptomyces gilvigriseus sp. nov., a novel actinobacterium isolated from mangrove forest soil. Antonie van Leeuwenhoek 2015;107: 1369– 1378 [CrossRef]
    [Google Scholar]
  13. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16: 313– 340 [CrossRef]
    [Google Scholar]
  14. Mo P, Zhao J, Li K, Tang X, Gao J et al. Streptomyces manganisoli sp. nov., a novel actinomycete isolated from manganese-contaminated soil. Int J Syst Evol Microbiol 2018;68: 1890– 1895 [CrossRef]
    [Google Scholar]
  15. Atlas RM. Parks LC. editor Handbook of Microbiological Media Boca Raton, FL: CRC Press; 1993
    [Google Scholar]
  16. Ridgway R. Color Standards and Color Nomenclature Washington, DC: Published by the author; 1912; pp 1– 43
    [Google Scholar]
  17. Jin L, Zhao Y, Song W, Duan L, Jiang S et al. Streptomyces inhibens sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.). Int J Syst Evol Microbiol 2019;69: 688– 695 [CrossRef]
    [Google Scholar]
  18. LH X, WJ L, Liu ZH, Jiang CL. Actinomycetes Systematics: Principles, Methods and Practices Beijing, China: Science Press; 2007
    [Google Scholar]
  19. Hallander HO, Laurell G. Identification of cephalosporin-resistant Staphylococcus aureus with the disc diffusion method. Antimicrob Agents Chemother 1972;1: 422– 426 [CrossRef]
    [Google Scholar]
  20. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29: 319– 322 [CrossRef]
    [Google Scholar]
  21. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970;20: 435– 443 [CrossRef]
    [Google Scholar]
  22. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977;100: 221– 230 [CrossRef]
    [Google Scholar]
  23. Kroppenstedt RM. Fatty acid and menaquinone analysis of actinomycetes and related organisms In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London, England: Academic Press; 1985; pp 173– 199
    [Google Scholar]
  24. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2: 233– 241 [CrossRef]
    [Google Scholar]
  25. Yoon S-H, Ha S-min, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110: 1281– 1286 [CrossRef]
    [Google Scholar]
  26. KQ L, Tang XK, Zhao JR, Guo YH, Tang YJ et al. Streptomyces cadmiisoli sp. nov., a novel actinomycete isolated from cadmium-contaminated soil. Int J Syst Evol Microbiol 2019;69: 1024– 1029
    [Google Scholar]
  27. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67: 1613– 1617 [CrossRef]
    [Google Scholar]
  28. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33: 1870– 1874 [CrossRef]
    [Google Scholar]
  29. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4: 406– 425
    [Google Scholar]
  30. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17: 368– 376 [CrossRef]
    [Google Scholar]
  31. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969;18: 1– 32 [CrossRef]
    [Google Scholar]
  32. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16: 111– 120 [CrossRef]
    [Google Scholar]
  33. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016;32: 929– 931 [CrossRef]
    [Google Scholar]
  34. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14: 60 [CrossRef]
    [Google Scholar]
  35. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33: 152– 155
    [Google Scholar]
  36. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68: 461– 466 [CrossRef]
    [Google Scholar]
  37. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106: 19126– 19131 [CrossRef]
    [Google Scholar]
  38. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37: 463– 464
    [Google Scholar]
  39. Muramatsu H, Nagai K. Streptomyces tsukubensis sp. nov. a producer of the immunosuppressant tacrolimus. J Antibiot 2013;66: 251– 254
    [Google Scholar]
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