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Abstract

A Gram-stain-positive actinobacterial strain, designated YJ01, was isolated from a spinach farming field soil at Shinan in Korea. Strain YJ01 was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5–1.9 µm, and was able to grow at 10–37 °C (optimum, 28–30 °C), at pH 4.5–9.0 (optimum, pH 7.0–8.0) and at salinities of 0–7.5 % (w/v) NaCl (optimum, 1.0 % NaCl). Sequence similarities of the 16S rRNA gene of strain YJ01 with closely related relatives were in the range 96.2–92.8 %, and the results of phylogenomic analysis indicated that strain YJ01 was clearly separated from species of genera in the family showing average nucleotide identity values of 84.2–83.4 %. The predominant isoprenoid quinone was identified as MK-8(H) and the major fatty acids were iso-C , iso-C  h, iso-C and anteiso-C ω. The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was -Orn–Gly–d-Glu. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid. The G+C content of the genome was 70.1 mol%. On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01 is considered to represent a novel species of a new genus in the family , for which the name gen. nov. sp. nov. is proposed. The type strain of is YJ01 (=KACC 19547=NBRC 113173).

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2020-01-17
2020-02-28
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