1887

Abstract

To date, there is sparse information for the genus with CC-AMO-30D as the only described member. We report here a new species isolated from mangrove soil collected at Malaysia Tanjung Piai National Park and perform polyphasic characterization to determine its taxonomic position. Strain CL23 is a Gram-negative, yellow-pigmented, strictly aerobic, catalase-positive and oxidase-positive bacterium. The optimal growth conditions were determined to be at pH 7.0, 30–37 °C and in 1–2 % (w/v) NaCl. The major respiratory quinone was menaquinone-6 (MK-6) and the highly abundant polar lipids were four unidentified lipids, a phosphatidylethanolamine and two unidentified aminolipids. The 16S rRNA gene similarity between strain CL23 and CC-AMO-30D is 96.67 %. Strain CL23 and CC-AMO-30D clustered together and were distinguished from taxa of closely related genera in 16S rRNA gene phylogenetic analysis. Genome sequencing revealed that strain CL23 has a genome size of 4.4 Mbp and a G+C content of 40.72 mol%. Overall genome related indexes including digital DNA–DNA hybridization value and average nucleotide identity are 17.70 % and approximately 70%, below the cutoffs of 70 and 95%, respectively, indicated that strain CL23 is a distinct species from CC-AMO-30D. Collectively, based on the phenotypic, chemotaxonomic, phylogenetic and genomic evidences presented here, strain CL23 is proposed to represent a new species with the name sp. nov. (KCTC 72252=LMG 31418). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/P025501/1)
    • Principle Award Recipient: Neil C. Bruce
  • Slovenian Research Agency (Award P4-0097 (C))
    • Principle Award Recipient: Maša Vodovnik
  • Universiti Teknologi Malaysia (Award 20H43)
    • Principle Award Recipient: Chun Shiong Chong
  • Ministry of Education Malaysia (Award 4B297)
    • Principle Award Recipient: Chun Shiong Chong
  • Biotechnology and Biological Sciences Research Council (Award BB/P027717/1)
    • Principle Award Recipient: Neil C. Bruce
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2020-01-24
2024-03-29
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