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Volume 70, Issue 4

sp. nov., isolated from gut of larva of Free

Abstract

A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13, was isolated from gut of the larva of collected from Wanju-gun, South Korea. The growth range of NaCl concentration was 0–3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10–40 °C (optimally 28–30 °C), and the pH range for growth was pH 6.0–9.0 (optimally pH 7.0–8.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13 had a high sequence similarity to members of the genus , having the highest similarity with DSM 19459 (97.7 %), CHO1 (97.1 %), and SK 18 (97.0 %), and formed a distinct lineage with DSM 19459 within the genus . A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree. The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were -C, -C and -C. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, an inidentified glycolipid and an unidnetified lipid. The peptidoglycan type was supposed to be the B2 with amino acids -alanine, -glutamic acid, glycine, -homoserine and -ornithine. The genomic DNA G+C content was 68.0 mol%. Based on the polyphasic taxonomic data, strain DFW100M-13 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is DFW100M-13 (=KACC 19323=NBRC 113120).

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Keyword(s): menaquinone , Microbacteriaceae , Microbacterium protaetiae , peptidoglycan and Protaetia brevitarsis seulensis
© 2020 The Authors
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2020-02-13
2024-04-19
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Microbacterium protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis
Int J Syst Evol Microbiol 70, 2226 (2020); https://doi.org/10.1099/ijsem.0.003967
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