1887

Abstract

A novel thermophilic bacterium, designated CFH 72773 was isolated from the enrichment of a Jinze hot spring sample which was collected from Dientan town, Tengchong county, Yunnan province, south-western PR China. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and non-sporulating. The taxonomic position of the strain was investigated by using a polyphasic approach. Growth occurred at 37–75 °C, pH 6.0–8.0 and with 0–2.0 % (w/v) NaCl. Comparison of the 16S rRNA gene sequences indicated the strain represented a member of the genus and showed close relationships to the type strains YIM 77925 (96.3 % similarity) and RF-4 (96.2 % similarity). The whole genome of CFH 72773 consisted of 2.25 Mbp and the DNA G+C content was 69.5 mol%. A total of 2262 genes, including a variety of enzymes for chemolithotrophy and anerobic respiration, were predicted. The strain had a unique negative oxidase activity and could hydrolyze starch at high temperature. Furthermore, various genes related to methane, sulfur, fumarate and nitrate metabolism were found, all these indicated that it is worth studying the novel strain. The predominant menaquinone is MK-8. The predominant cellular fatty acids included iso-C, iso-C and iso-C. The major polar lipids were comprised of aminophospholipid, glycolipid and two phospholipids. On the basis of low ANI values, different phenotypic and chemotaxonomic characters and phylogenetic analysis, we made a proposal that strain CFH 72773 represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is CFH 72773 (=CCTCC AB2018244=KCTC 43129).

Funding
This study was supported by the:
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: Hong Ming
  • Jiangsu Agricultural Science and Technology Innovation Fund (Award CX-18 2016)
    • Principle Award Recipient: Chenlong Ding
  • Henan Province University youth researcher support project (Award 2017GGJS106)
    • Principle Award Recipient: Hong Ming
  • Innovation Scientists and Technicians Troop Construction Projects of Henan Province (Award CXTD2016043)
    • Principle Award Recipient: Guo-Xing Nie
  • Natural Science Foundation of China (Award 3150004)
    • Principle Award Recipient: Hong Ming
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2020-01-22
2024-12-14
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