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Volume 70, Issue 3

Genome-based analyses reveal a synonymy among Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996, Ventosa . 2004, Xu . 2007 and Cui . 2007. Emended description of Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996 Open Access

Abstract

A comparative taxonomic study of , , and was carried out using different approaches, 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), phylogenomic analysis based on the comparison of the core genome, orthologous average nucleotide identity (OrthoANI), Genome-to-Genome Distance Calculator (GGDC), synteny plots and polar lipid profile (PLP). The MLSA study, using the five concatenated housekeeping genes , , , and ′, and the phylogenomic analysis based on 1347 core translated gene sequences obtained from their genomes showed that JCM 9100, JCM 10247, JCM 13916 and JCM 13561 formed a robust cluster, clearly separated from the rest of species of the genus . The OrthoANI and digital DDH values, calculated by the GGDC, showed percentages among JCM 9100, JCM 10247, JCM 13916 and JCM 13561 that ranged from 98.1 to 97.5 %, and 84.0 to 78.0 %, respectively, while these values among those strains and the type strains of their most related species of were equal or lower than 90.8 and 41.2 %, respectively. Moreover, degree of synteny across the four genomes was very high, especially between the genomes of JCM 13561 and JCM 13916. In addition, the PLP is quite similar among the four strains studied, showing a common pattern typical of the neutrophilic species of the genus . Overall, these data show that , , and constitute a single species. Thus, the latter three should be considered as later, heterotypic synonyms of based on the rules for priority of names. We propose an emended description of , including the features of , and .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): emended description , Halorubrum , Halorubrum distributum , synonym and taxonomy
Funding
This study was supported by the:
  • Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía (Award BIO-213)
    • Principle Award Recipient: Antonio Ventosa
  • Ministerio de Ciencia, Innovación y Universidades (Award CGL2017-83385-P)
    • Principle Award Recipient: Antonio Ventosa
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-01-23
2024-04-24
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Genome-based analyses reveal a synonymy among Halorubrum distributum Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996, Halorubrum terrestre Ventosa et al. 2004, Halorubrum arcis Xu et al. 2007 and Halorubrum litoreum Cui et al. 2007. Emended description of Halorubrum distributum Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996
Int J Syst Evol Microbiol 70, 1698 (2020); https://doi.org/10.1099/ijsem.0.003956
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