1887

Abstract

A novel bacterium, XHU 5135, belonging to the genus , was isolated from a salt lake sample collected in Xinjiang Province, north-west PR China. The isolate was Gram-stain-negative, rod-shaped and non-motile. The strain was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 5–25 % (optimum, 10–13 %), at 13–41 °C (35–37 °C) and at pH 6.0–10.0 (pH 7.0–8.0). The predominant ubiquinone was Q-9. The major fatty acids were C cyclo 8 and C. The G+C content of the genomic DNA was 58.1 mol%. The affiliation of strain XHU 5135 with the genus was confirmed by 16S rRNA gene sequence comparisons. The closest type strain was YIM 90637, which showed a 16S rRNA gene sequence similarity of 97.5 %. The ANI value between XHU 5135 and the closest type strain was 80.01 %. The estimated digital DNA–DNA hybridization estimate value between strain XHU 5135 and the closest type strain was 22.80 %. Phenotypically, the characteristics of XHU 5135 were shown to differ from the most closely related species, . On the basis of the data from this polyphasic study, strain XHU 5135 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain XHU 5135 (=CCTCC AB 2016344=KCTC 42945=DSM 104700).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30660005)
    • Principle Award Recipient: Tong-Wei Guan
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2020-01-07
2024-03-29
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References

  1. Wang Y, Tang S-K, Lou K, Lee J-C, Jeon CO et al. Aidingimonas halophila gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol 2009; 59:3088–3094 [View Article]
    [Google Scholar]
  2. Guan T-W, Xiao J, Zhao K, Luo X-X, Zhang X-P et al. Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake. Int J Syst Evol Microbiol 2010; 60:349–352 [View Article]
    [Google Scholar]
  3. Cerny G. Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. European J. Appl. Microbiol. Biotechnol. 1978; 5:113–122 [View Article]
    [Google Scholar]
  4. Smibert RM, Krieg NR. General characterization. In Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA. (editors) Manual of Methods for General Bacteriology Washington: American Society for Microbiology; 1981 pp 409–443
    [Google Scholar]
  5. Zhu XF. Modern Experimental Technique of Microbiology Zhejiang University Press, Hangzhou English translation; 2011
    [Google Scholar]
  6. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  7. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  8. Mata JA, Martínez-Cánovas J, Quesada E, Béjar V. A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 2002; 25:360–375 [View Article]
    [Google Scholar]
  9. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article]
    [Google Scholar]
  10. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997; 47:1129–1133 [View Article]
    [Google Scholar]
  11. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  12. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newwark: MIDI Inc; 1980
    [Google Scholar]
  13. Li W-J, Xu P, Schumann P, Zhang Y-Q, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article]
    [Google Scholar]
  14. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
    [Google Scholar]
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article]
    [Google Scholar]
  17. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  18. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
    [Google Scholar]
  19. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
    [Google Scholar]
  20. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  21. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  22. He L, Li W, Huang Y, Wang L, Liu Z et al. Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 2005; 55:1939–1944 [View Article]
    [Google Scholar]
  23. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on systematic bacteriology. Report of the AD hoc Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Bacteriol 1987; 37:463–464
    [Google Scholar]
  24. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article]
    [Google Scholar]
  25. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article]
    [Google Scholar]
  26. Zhi X-Y, Jiang Z, Yang L-L, Huang Y. The underlying mechanisms of genetic innovation and speciation in the family Corynebacteriaceae: a phylogenomics approach. Mol Phylogenet Evol 2017; 107:246–255 [View Article]
    [Google Scholar]
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