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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov., isolated from shrimp gill from the Okinawa Trough Free

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, long, straight-rod and non-flagellated marine bacterium strain, designated SCR12, was isolated from the gill of a shrimp collected in the Tangyin hydrothermal field of the Okinawa Trough. The growth temperature was in the range of 16–40 °C and the optimum temperature was 37 °C. Optimal growth occurred at pH 6.5 and in the presence of 3 % (w/v) NaCl. The predominant isoprenoid quinone of strain SCR12 was menaquinone-6 (MK-6). The predominant fatty acids (>10 %) were iso-C (44.2 %), iso-C G (19.0 %) and iso-C 3-OH (12.1 %). The major polar lipids comprised one phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminolipids and three unidentified lipids. Based on the results of 16S rRNA gene sequence analysis, strain SCR12 was found to be most closely related to CL-SS4 (98.09 %), followed by BB-My12 (97.65 %), SW-63 (97.58 %) and DSM 13258 (97.31 %) and with lower sequence similarities (95.74–97.10 %) to other species of the genus . Genome relatedness between strain SCR12 and CL-SS4 was computed using both average nucleotide identity (ANI) and DNA–DNA hybridization (DDH) and resulted in values of 85.6 % and 29.3±2.3 %, respectively. The genomic DNA G+C content of strain SCR12 was 42.3 mol%. On the basis of polyphasic analysis, the strain SCR12 was considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is SCR12 (=MCCC 1K03731=JCM 33425).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): hydrothermal field , Muricauda alvinocaridis , Okinawa Trough and shrimp gill
Funding
This study was supported by the:
  • Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology (Award 2016ASKJ14)
    • Principle Award Recipient: Min Yu
  • The Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao) (Award LMEES-YTSP-2018-02-07)
    • Principle Award Recipient: Min Yu
  • National Natural Science Foundation of China (Award U1706208)
    • Principle Award Recipient: Min Yu
  • National Key R&D Program of China (Award 2018YFC0310701)
    • Principle Award Recipient: Xiao-Hua Zhang
© 2020 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: sp. nov., isolated from shrimp gill from the Okinawa Trough
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2020-01-07
2024-04-25
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Muricauda alvinocaridis sp. nov., isolated from shrimp gill from the Okinawa Trough
Int J Syst Evol Microbiol 70, 1666 (2020); https://doi.org/10.1099/ijsem.0.003953
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